荷叶总黄酮的提取工艺研究  

Research on the Extraction Technology of the Total Flavonoids from Lotus Leaf

作　　者：薛晓丽[1] 

机构地区：[1]吉林农业科技学院实验管理中心,吉林吉林132101

出　　处：《安徽农业科学》2009年第12期5500-5501,5503,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为荷叶的大规模开发利用提供技术保证。[方法]采用超声波辅助萃取法对荷叶总黄酮进行提取，并用分光光度法测定其含量，通过单因素及正交试验确定最佳提取工艺。[结果]当乙醇浓度达到60％时，总黄酮收率最大；液料比在10：1-30：1时，黄酮的收率随液料比的增大而增加较快，当液料比大于40：1后，黄酮收率趋于恒定；当作用时间为35min时，黄酮收率最大，但此时与30min相比，仅略微增加；提取时适宜的温度应为40℃；影响荷叶总黄酮收率的主次因素顺序为乙醇浓度〉超声时间〉液料比〉超声温度。[结论]荷叶总黄酮最佳提取工艺：将荷叶干粉按35：1的液料比加入浓度60％乙醇水溶液，浸泡24h，在超声波清洗槽中超声提取35min，超声温度35℃。在该条件下，荷叶总黄酮收率为13．68％。[Objective] The technical support for the development and utilization of lotus leaf ata large-scale was provided. [Method]The total flavonoids in lotus leaf were extracted with the ultrasound-assisted extraction method; its content was deter mined with spectrophotometric method and the optimum extraction process was studied through the single factor and orthogonal experiment. [Results] When the ethanol concentration was up to 60% , the largest yield of total flavonoids could be extracted; when the ration of the solution to raw material was from 10：1 to 30：1 , the yield of flavonoids was increased with the ratio-increasing of the solution to raw material and when the ration was at 40：1 , the yield of flavonoids tended to being stable. When the was 35 min, the maximum yield of flavonoids could be extracted, but compared with the treating time of 30 min, its yield was only slightly increased. The appropriate extracting temperature should be at 40 ℃ . The order of the factors impacting extracting yield of total flavonoids was ethanol concentration 〉 ultrasonic time 〉 liquid ratio 〉 ultrasonic temperature. [Conclusion] The best extracting process of total flavones from lotus leaf was： the ratio of lotus leaf powder to solution,35 ：1; the ethanol concentration,60%; the immersion time,24 h; the extracting time,35 min and temperature,35 4C,so that the yield of total flavonoids extracted from lotus leaf was 13.68%

关 键 词：总黄酮 超声波辅助萃取 荷叶 

分 类 号：Q946.91[生物学—植物学]