葛根素的分离与鉴定  

Isolation and Identification of Puerarin

作　　者：崔颖[1] 梁剑平[1] 郭延生[2] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所中国农业科学院新兽药工程重点开放实验室,甘肃兰州730050 [2]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《安徽农业科学》2009年第13期5817-5818,5882,共3页Journal of Anhui Agricultural Sciences

基　　金：甘肃省科技攻关项目(970614)

摘　　要：[目的]筛选出适于工业化生产葛根素的提取、分离、纯化工艺路线。[方法]用熔点、红外光谱、核磁氢谱和紫外分光光度法分析鉴定提取的葛根素精品。[结果]葛根素精品的熔点为327～328℃，与葛根素标准品的熔点基本相同；在薄层色谱上，精品与标准品的斑点在紫外灯下均显蓝色荧光，Rf值均为0．31；精品的红外数据为IRUMax^Kbc（cm^-1）：3398、1630、1584、1515、1443、1384、1257、1178、1118、1081、10141，标准品的红外数据为IRUmax^KBr（cm^-1）：3370、1631、1607、1514、1447、1396、1273、1210、1103、1059、1008；精品的核磁氢谱数据与文献报道相符；在250nm处测定精品的吸光度，由回归方程计算出精品的葛根素含量为97％，液-液萃取法的葛根素精品的产率为1．41％。[结论]液-液萃取法分离纯化葛根素成本低，操作简单，可用于工业化生产。[Objective] The purpose of the study was to screen out the process route of extracting, isolating and purifying puerarin, which was suitable for industrial production. [Method] Tne extracted and refined puemrin was analyzed and identified by melting point method, infrared spectrophotometry,nuclear magnetic resonance hydrogen （NMR-H） spectroscopy and UV speetrophotometry. [ Result] The melting point of refmed puerarin was 327 - 328℃ and it was basically identical with the mehing point of standard puerarin. On thin layer spectrum, both the dots of refined and standard puerarin showed were IRUMax^Kbc（cm^-1） ： 3 398, 1630, IRUMax^Kbc（cm^-1） ： 3 398, 1630, blue fluorescence under UV-light lamp and both of their P,f values were 0.31. were IRUMax^Kbc（cm^-1） The infrared data of refmed puerarin KBr - ： 3 398, 1 630, 1584, 1515, 1443, 1384, 1257, 1 178, 1 118, 1081andl041 and that of standard puerarin was IRUMax^Kbc（cm^-1）3 370, 1 631, 1 607, 1 514,1 447, 1 396, 1 273, 1 210, 1 103, 1 059 and 1 008.The NMR-H data of refined puerarin accorded with literature reports. The absorbance of refined puerarin was detected at 250 nm, the puerarin content of the refined was calculated to be 97% through regression equation.The yield rate of refined puerarin of liquid-liqiud extraction was 1.41 %. [Conclusion] Isolation and purification of puerarin by liquid-liqiud extraction was cheap in cost, simple to op-emte and could be used in industrial production.

关 键 词：野葛根 葛根素 提取 分离 

分 类 号：Q946.91[生物学—植物学]