绵羊肝脏生长激素受体基因表达的发育性变化研究  

Developmental Changes of GHR mRNA Expression Level in Sheep Liver

作　　者：黄治国[1,2] 谢庄[2] 

机构地区：[1]四川理工学院酿酒生物技术及应用四川省重点实验室,四川自贡643000 [2]南京农业大学动物科技学院,江苏南京210095

出　　处：《安徽农业科学》2009年第13期5894-5896,5942,共4页Journal of Anhui Agricultural Sciences

基　　金：新疆生产建设兵团博士基金(2003-02)

摘　　要：[目的]研究哈萨克羊和新疆细毛羊肝脏生长激素受体（Growth hormone receptor，G积）基因在发育早期的表达，为绵羊早期生长发育的研究提供资料。[方法]采用实时定量PCR检测2个品种羊在2、30、60、90和120日龄时肝脏GHR mRNA的水平，并用SPSS软件进行统计分析。[结果]绵羊肝脏GHR基因的表达量在2日龄时较高，30日龄时降到最低点，然后持续上升；30日龄后的变化趋势与绵羊的累积生长曲线呈正相关（P〈0．05）；哈萨克羊的GHR表达量在2～90日龄期间均低于新疆细毛羊，但仅在2和90日龄时差异极显著（P〈0．01）。[结论]雄性哈萨克羊和新疆细毛羊肝脏GHRmRNA表达的发育性变化模式基本相似，品种间差异较小；肝脏GHR基因在绵羊生长发育的调控中可能起着重要作用。[ Objective ] To detect the expression of liver growth hormone receptor gene （GHR） in early development of Kazak sheep and Xinjiang finewool sheep, so as to provide information for the research about the early growth and development of sheep. [Method] With real-time quantitative PCR, the liver GHR mRNA level was separately detected in two varieties of sheep at 2, 30, 60, 90 and 120 days old. Then the data was analyzed with SPSS software. [Result] The liver GHR gene was highly expressed in 2-day-old sheep, but the expression level fell to the lowest point at 30 days old and then rose continuously; the change trend after 30 days old was positively correlated with the cumulative growth curves of sheep （ P 〈 0.05） ; GHR mRNA level was lower in Kazak sheep than in Xinjiang fine-wool sheep during 2 - 90 days old, but the difference was extremely significant （ P 〈 0.01 ） only at 2 days old and 90 days old. [Conclusion] The male Kazak sheep and Xinjiang free-wool sheep have similar model of developmental changes of liver GHR mRNA arid the differences are smaller between these two species; GHR gene may play an important role in the regulation of sheep growth.

关 键 词：绵羊 肝脏 生长激素受体基因 表达 

分 类 号：S858.26[农业科学—临床兽医学]