野牛草种子蛋白质组双向电泳样品制备方法的分析比较  

Comparative Analysis on the Preparation Methods of Bidirectional Electrophoretic Samples of Proteome from Buffalo Grass Seeds

作　　者：孙杰[1,2,3] 刘永志[2] 康俊梅[3] 熊军波[3] 沈静[4] 吕会刚[3] 孙彦[5] 

机构地区：[1]内蒙古农业大学,内蒙古呼和浩特010018 [2]内蒙古农牧业科学院,内蒙古呼和浩特010030 [3]中国农业科学院北京畜牧兽医研究所,北京100193 [4]甘肃农业大学,甘肃兰州730070 [5]中国农业大学,北京10019

出　　处：《安徽农业科学》2009年第13期5982-5984,共3页Journal of Anhui Agricultural Sciences

基　　金：中国农业科学院北京畜牧兽医研究所所长基金项目

摘　　要：[目的]比较野牛草种子蛋白质的提取方法。[方法]以"中坪一号"野牛草种子为试材,分别采用TCA/A(三氯乙酸/丙酮)沉淀法和Trizol法提取种子蛋白,并利用条件一致的蛋白质双向电泳体系进行分析比较。[结果]TCA/A法提取的蛋白质双向电泳图谱背景噪音大,清晰度差,横向条纹较多,图谱上呈现多个形状不规则的斑点,杂色与正常蛋白质点有区别,对其他蛋白质点构成高度覆盖和遮避,影响图谱信息的准确表达;Trizol法所提取的蛋白质样品,其双向电泳图谱背景噪音小,横向条纹少,非蛋白类杂质少,无斑点间的遮避现象,蛋白质点数量增多,为野牛草种子蛋白质组定量、定性分析提供了丰富的信息。[结论]与TCA/A沉淀法相比,改进的Trizol法是野牛草种子蛋白质提取的适用方法。[Objective] The aim of the study was to compare the methods of extracting protein from buffalo grass seeds. [Method] With seeds of buffalo grass Zhongping 1 as tested materials, the proteins were extracted from seeds by TCA/A precipitation and Trizol method and analyzed and compared by bidirectional electrophoresis system with uniform conditions of protein. [ Result] The background noise of bidirectional electrophoresis map of protein extracted by TCA/A method was greater, its clarity was poor, its horizontal striations were more and there were many spots with irregular shape appeared on it. The variegated spots had difference from the normal protein spots, they formed high coverage and shading on the other protein spots and affected the accurate expression of map information. The background noise of bidirectional electrophoresis map of protein extracted by Trizol method was little, its horizontal striations and non - protein impurities were less, there was no shading phenomenon among spots and more protein spots, so it provided abundant information for quantitative and qualitative analyses on the proteome from buffalo grass seeds. [Conclusion] Compared with TCA/A precipitation, the ira. proved Trizol method was suitable for extracting protein from buffalo grass seeds.

关 键 词：野牛草 种子蛋白质组 蛋白质双向电泳 图谱 

分 类 号：S188[农业科学—农业基础科学]