运输温度与时间对猪孤雌胚胎发育的影响  

Effects of Different Transport Temperature and Transport Time on Development of Porcine Parthenogenetic Embryo

作　　者：张卫红[1,2] 闫军[2] 冯冲[1,2] 龙川[2] 张兆旺[1] 潘登科[2] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]中国农业科学院北京畜牧兽医研究所,畜禽遗传资源与利用农业部重点实验室,北京100193

出　　处：《安徽农业科学》2009年第13期5998-5999,6013,共3页Journal of Anhui Agricultural Sciences

基　　金：国家"863"项目(2006AA02Z113);国家基本科研业务费(ywf-td-1);中国农业科学院院长基金资助

摘　　要：[目的]比较不同运输温度与时间对猪孤雌胚胎发育的影响,确立猪克隆胚胎的最佳运输温度与时间。[方法]研究不同温度(37、38、39℃)对孤雌胚胎卵裂率、囊胚率的影响;不同时间(2、3、4 h)对孤雌胚胎卵裂率、囊胚率的影响。[结果]培养3 h后,在39℃运输时,孤雌胚胎的卵裂率和囊胚率与37℃、38℃的卵裂率和囊胚率相比,差异显著(P<0.05);培养3 h后,孤雌胚胎的卵裂率和囊胚率与培养2 h、4 h的卵裂率和囊胚率相比,差异显著(P<0.05)。[结论]在39℃,3 h内进行孤雌胚胎的运输,能获得较高的卵裂率、囊胚率。[ Objective] The research aimed to establish the best transport temperature and transport time of porcine parthenogenetic embryo clone. [ Methed]The effects of different temperatures （37, 38, 39 ℃ ） on parthenogenetic embryo cleavage and blastocyst rate and the effects of different time （2, 3, 4 h） on parthenogenetic embryo cleavage and blastocyst rate were studied. [ Result ]There were statistical differences at 0.05 level of the parthenogenetic cleavage rate and blastocyst rate between development of parthenogenetic embryos at 39, 37 and 38 ℃ .And there were statistical differences at 0.05 level of the parthenogenetic cleavage rate and blastocyst rate between development of parthenogenetic embryos in 3, 2 and 4 h. [ Conclusion]This study suggested that carrying out the transport of parthenogenetic embryos at 39 ℃, within 3 h could obtain a higher cleavage rate and blastecyst rate.

关 键 词：猪 运输时间 运输温度 孤雌胚胎 卵裂率 囊胚率 

分 类 号：S828[农业科学—畜牧学]