一串红菌核病病原鉴定及其主要生物学特性研究  

Studies on the Identification and the Major Biological Characteristic of the Pathogen of Sclerotinia Rot on Salvia splendens

作　　者：陈永强[1] 李梅[1] 牟登科[1] 沈志华[1] 杨秀楷 曾大兴[3] 

机构地区：[1]电子科技大学中山学院化学与生物系,广东中山528402 [2]中山市横栏镇农业服务中心,广东中山528402 [3]深圳职业技术学院应用化学与生物技术学院,广东深圳518055

出　　处：《安徽农业科学》2009年第13期6067-6071,共5页Journal of Anhui Agricultural Sciences

摘　　要：[目的]明确引起广东省中山市一串红茎基部腐烂的病原菌种类及其主要生物学特性。[方法]对一串红病株进行了病原菌分离纯化，研究了其形态特征、致病性、主要生物学和分子生物学特性。[结果]该菌在多种培养基上均可生长；生长温度范围为5～30℃，最适生长温度为加～25℃。在供试碳源中，病原菌对葡萄糖利用最好；在供试的氮源中，对硫酸铵利用最好；菌丝在pH2—9均可生长，生长的最佳pH值为3～6；同时筛选出几种对病原菌有明显抑制作用的杀菌剂。[结论]引起一串红茎基腐菌核病的病原菌鉴定为核盘菌，这在中国大陆首次报道。[Objective] The aim of the study was to confirm the species and the major biological characteristics of the pathogen of scleretinia rot on Salvia splenden in Zhongshan City, Guangdong Province. [Method] The pathogen of sclerotinia rot on Salvia splendens was purified, the morphology, pathogenicity, the major biological and molecular biological characteristics of the pathogen were studied. [Result] The study showed that the pathogen could grow in several kinds of culture media. The temperature for mycelium growth ranged from 5 to 30℃ and the optimum growth temperature ranged from 20 to 25 ℃ ; among the tested carbon and nitrogen sources, glucose was the most useful carbon type, ammnium sulfate was the most favorable nitrogen source for mycelium growth; mycelium could grow at pH 2 to 9, and the optimum ranged from 3 to 6. Several fungicides were selected as inhibitor to mycelium growth. [Conclusion] It was the first report in mainland of China that Sclerotinia sclerotiorum （Lib.） de Bary was identified as the pathogen to sclerotinia rot on ＆tlvia splendens.

关 键 词：一串红 菌核病 病原鉴定 生物学特性 

分 类 号：S182[农业科学—农业基础科学]