马尾松花粉多糖及其酯化物对离体蟾蜍心肌生理特性的影响  

Effects of Pine Pollen Polysaccharide and Its Sulfate on the Physiological Characteristics of Isolated Toad Cardiac Muscle

作　　者：夏瑜[1] 石丽花 刘媛[1] 刘志河 耿越[1] 

机构地区：[1]山东师范大学生命科学学院动物抗性生物学重点实验室,济南250014 [2]烟台新时代健康产业有限公司,烟台264006

出　　处：《天然产物研究与开发》2009年第2期231-234,共4页Natural Product Research and Development

摘　　要：利用常规离体蟾蜍心脏灌流法,通过生理记录仪经张力换能器系统描记心脏活动,观察了60%马尾松花粉多糖及其硫酸酯化多糖对离体蟾蜍心肌收缩力及心率的影响,并用普萘洛尔、氯化镉、维拉帕米等药物对心肌上的特异受体进行阻断,初步探讨了酯化多糖对心肌的作用机制。结果表明:60%多糖有降低离体蟾蜍心肌收缩力的作用,酯化60%多糖则有增强心肌收缩力的作用,两者对心率都没有影响;酯化60%多糖可以逆转普萘洛尔、氯化镉对心肌的抑制作用,而在维拉帕米存在的情况下,其正性肌力作用受到抑制,说明酯化多糖的正性肌力作用可能是通过胞膜上的L型-钙通道介导完成,而与β1受体无关。因而,加深研究酯化多糖影响心脏生理活动的的分子机制,对治疗相关疾病和开发松花粉多糖具有双重意义。By means of perfusing apparatus of isolated toad heart, we observed the effects of 60% alcohol precipitated Piruts massoniana pollen polysaccharide（PM60） and its sulfate（PM60S） on the contractility of toad cardiac muscle. The activities of heart muscle were recorded by tonotransducer and BL420 recorder. By adding drugs like propranolol（ Pro）, CdCI2 and verapamil（Ver） to block the specialized receptor on cardiomyocytes, we discussed the probable effecting mechanism of PM60S on cardiomyocytes. The results showed that PM60 could inhibit myocardial contractility; PM60S could significantly strengthen the myocardial contractility. Both of them had no effect on heart rate. PM60S could reverse the inhibition activity of Pro and CdC12. But in the presence of Ver,the positive inotropic effect of PM60S was inhibited completely. It was proved that the positive inotropic effect of PM60S may work through L-type Ca^2＋ channel,and be independent of the B1-receptor.

关 键 词：马尾松花粉多糖 酯化多糖 蟾蜍 离体心脏灌流 心肌收缩力 

分 类 号：Q463[生物学—生理学] R285[医药卫生—中药学]