麦红吸浆虫成虫和幼虫滞育过程中蜕皮激素的定量分析  

Quantitative analysis of ecdysteroid in adults and the pre-diapause,diapause and post-diapause larvae of wheat blossom midge,Sitodiplosis mosellana Gehin

作　　者：成卫宁[1] 李建军[1] 李怡萍[1] 李修炼[1] 仵均祥[1] 王洪亮[2] 

机构地区：[1]西北农林科技大学植物保护学院,植保资源与病虫害治理教育部重点实验室,陕西杨凌712100 [2]河南科技学院植物保护系,新乡453003

出　　处：《植物保护学报》2009年第2期163-167,共5页Journal of Plant Protection

基　　金：教育部重点项目(105165);国家自然科学基金项目(30370936);国家“十一五”科技支撑计划(2006BAD08A05)

摘　　要：为明确麦红吸浆虫成虫和幼虫滞育过程中体内蜕皮激素的变化规律,采用放射免疫分析法测定了麦红吸浆虫成虫和幼虫滞育过程中的蜕皮激素滴度。结果表明,同一天羽化的成虫,雌虫蜕皮激素滴度为7.43 pg头/,显著高于雄虫;滞育前后及滞育期幼虫蜕皮激素滴度存在显著差异,滞育前为5.22 pg头/,进入滞育后显著降低;滞育期间,冬季蜕皮激素滴度为6.64～7.10 pg头/,显著高于其它季节;滞育解除后蜕皮激素滴度显著降低,之后随着幼虫的生长发育逐渐升高,至化蛹前约1周达到最高,为15.22 pg头/。滞育期间同期裸露幼虫与结茧幼虫,以及不同滞育年限幼虫的蜕皮激素滴度差异均不显著。In order to understand the roles of ecdysteroid in adults and diapausing larvae of wheat blossom midge, Sitodiplosis mosellana Gehin, radioimmunoassay was firstly used to analyze the ecdysteroid titers in adults, pre-diapause, diapause and post-diapause larvae of wheat blossom midge. For adults emerged from pupae in the same day, the ecdysteroid titer in female （7.43 pg/individual） was significantly higher than that in male （0.15 pg/individual）. The ecdysteroid titer in/among pre-diapause, diapause and post-diapause larvae showed significantly different. The ecdysteroid titer was 5.22 pg/individu- al in pre-diapause larvae and then decreased significantly after entering diapause. It was 6.64 -7.10 pg/ individual in over-wintering diapause larvae, significantly higher than those in other stages during diapause. It reduced significantly after diapause termination, and increased gradually with the development and reached a peak, making up 15.22 pg/individual about one week before pupation. No obvious difference of ecdysteroid titers was found between 1^ st and 2^nd year of diapause larvae, as well as between no-cocooned and cocooned larvae in the same diapause stages.

关 键 词：麦红吸浆虫 蜕皮激素 放射免疫法 

分 类 号：S435.122[农业科学—农业昆虫与害虫防治]