斜纹夜蛾对茚虫威的抗性机制  

Resistance mechanisms of Spodoptera litura to indoxacarb

作　　者：王建军[1] 董红刚[1,2] 袁林泽[1] 

机构地区：[1]扬州大学园艺与植物保护学院,扬州225009 [2]扬州市邗江区植物保护站,扬州225009

出　　处：《植物保护学报》2009年第2期168-172,共5页Journal of Plant Protection

基　　金：江苏省基础研究计划(自然科学基金)(BK2008211)

摘　　要：以斜纹夜蛾对茚虫威相对敏感种群和抗性倍数为15.63倍的抗性选育种群为材料,通过解毒酶活性测定与钠离子通道基因片段的克隆、测序,研究了斜纹夜蛾对茚虫威的抗性机制。结果表明,与相对敏感种群相比,抗性种群酯酶活性提高了2.27倍,但抗性和敏感种群谷胱甘肽S-转移酶和多功能氧化酶O-脱甲基活性无显著差异;3龄幼虫酯酶活性随着抗性指数的上升而逐渐提高;从相对敏感和抗性斜纹夜蛾种群3龄幼虫基因组DNA中扩增出位于钠离子通道IIS5-IIS6的341 bp DNA片段;与相对敏感种群相比,茚虫威抗性种群钠离子通道基因没有发生突变。The resistance mechanisms of indoxacarb-selected population of common cutworm, Spodoptera litura （Fabricius） , with resistance ratio of 15.63, were investigated by enzyme assays of esterases （ESTs）, glutathione S-transferases （GSTs）and microsomal muhifunctional oxidase （MFO）and cloning and sequencing of sodium channel gene. The results showed that the activity of ESTs increased by 2.27-fold compared with the susceptible population, while the activities of MFO and GSTs had no significant difference. Positive correlation was observed between ESTs activity in 3rd larvae of every selected generation and resistance ratio. DNA fragments of 341 bp in length in the IIS5-IIS6 region of sodium channel paraorthologous gene were amplified and sequenced in both resistant and susceptible population, no sodium channel mutation was found in the resistant population compared with the susceptible population.

关 键 词：斜纹夜蛾 茚虫威 抗性机制 酯酶 钠离子通道 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]