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作 者:段敏[1] 王金信[1] 贺红武[2] 彭学岗[1] 彭浩[2]
机构地区:[1]山东农业大学植物保护学院,泰安271018 [2]华中师范大学化学学院农药化学研究所,武汉430079
出 处:《植物保护学报》2009年第2期179-182,共4页Journal of Plant Protection
基 金:国家自然科学基金(20372023)
摘 要:采用培养皿生物测定方法研究化合物O,O-二甲基-1-(2,4-二氯苯氧基乙酰氧基)呋喃甲基膦酸酯(试验代号HIA-1)的生物活性及其对苘麻部分生化指标的影响。结果表明,化合物HIA-1在0.05~10 mg/L处理剂量下,对高粱、稗草、黄瓜、萝卜、苘麻和油菜的根、芽生长有不同程度的抑制作用,其中黄瓜、萝卜、苘麻和油菜较为敏感,10 mg/L处理后,根长、芽长抑制率分别在86.2%和88.2%以上;0.01 mg/L处理后对根、芽生长有明显的促进作用。0.1 mg/L处理后,苘麻幼根细胞存活率超过100%,表现出激活作用;随着浓度升高,细胞存活率逐渐降低。1 000 mg/LHIA-1处理时,苘麻幼苗及幼根SOD活性分别约为对照的50%和30%;当浓度分别为0.1和1 mg/L时,幼苗和幼根SOD活性高于对照。10~1 000 mg/L处理时幼苗和幼根POD活性低于对照,1000 mg/L时约为对照的58%和40%;低浓度时POD活性高于对照,1 mg/L时幼苗和幼根POD活性均约为对照的130%。The bioactivity of the novel compound, [ O, O-dimethyl-1- (2,4-dichlorophenoxy-acetyloxy) - methylfuran phosphonate ] (Symbol: HIA-1 ) and the influence on some biochemical indexes of Abutilon theophrasti were studied. The result showed that HIA-1 had different degree inhibitory effect to Sorghum moench, Echinochloa crusgalli, Cucumis sativus, Raphanus sativus, Abutilon theophrasti and Brassica campestris from 0.05 mg/L to 10 mg/L. Thereinto, Cucumis sativus, Raphanus sativus, Abutilon theo- phrasti and Brassica campestris were more sensitive, and the inhibitory rates of radicle and bud length were above 86.2% and 88.2% after treatment at 10 mg/L. But the radicle and bud were promoted at 0.01 mg/L. The cells comparative survival rate of radicle, which was reduced followed with increase of concentration, was above 100% at 0.1 rag/L, representing simulative effect in A. theophrasti. Superoxide dismutase (SOD) activities of seedling and radicle ofA. theophrasti were about 50% and 30% of control at 1000mg/L respectively, which were more than the control at 0.1 and 1 mg/L. Peroxidase (POD) activities of seedling and radicle of A. theophrasti were lower than the control activities from lO to 1 000 mg/L, with 58% and 40% of those of the control at 1000 mg/L, but higher than the control activities at lower concentrations with both about 130% of those of the control at 1 mg/L.
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