一起水痘爆发的病毒分离鉴定及血清学分析  

Isolation Identification and Serological Analysis on Varicella-zoster Virus from an Outbreak in Shanghai in 2007

作　　者：陆菁[1] 李崇山[1] 胡家瑜[1] 汤素文[1] 毛乃颖[2] 丁晓光[1] 刘敏勇[1] 檀晓娟[2] 徐敏钢 许文波[2] 

机构地区：[1]上海市疾病预防控制中心,上海200336 [2]中国疾病预防控制中心病毒病预防控制所世界卫生组织西太平洋区麻疹参比实验室,病毒基因工程国家重点实验室,北京100050 [3]上海市闸北区疾病预防控制中心,上海200072

出　　处：《中国疫苗和免疫》2009年第2期127-130,共4页Chinese Journal of Vaccines and Immunization

摘　　要：目的对上海市闸北区2007年一起水痘爆发进行病原学鉴定和血清学分析。方法对采集的双份血清用酶联免疫吸附试验进行水痘-带状疱疹病毒(Varicella-zoster Virus,VZV)IgM和IgG抗体检测,采用疫苗生产用的VZV敏感细胞--人胚肺成纤维细胞(MRC-5),对采集的疱疹液进行病毒分离以及标准血清中和鉴定,并通过特异性的引物对病毒分离株进行聚合酶链反应(Polymerase Chain Reaction,PCR)扩增。结果采集到的5例病人的双份血清中,急性期血清IgM阳性2份,IgG阳性1份,灰区2份;恢复期血清IgG均为阳性,其中4份标本IgG抗体滴度≥4倍升高。从采集的5份疱疹液标本中均分离到致细胞病变的病毒,经VZV标准血清中和鉴定,证实为VZV。PCR扩增产物电泳结果显示,在目的条带处均有单一的特异性条带。结论该次爆发是由VZV引起的,水痘减毒活疫苗(Varicella Virus Attenuated Live Vaccine,VarV)的作用已被证实,但该次水痘爆发中,47.4%的患者在出生后3年内曾接种过国产或者进口VarV,应深入研究VarV的免疫策略。Objective To make serological analysis and identify the etiology of an outbreak of chickenpox in Zhabei District of Shanghai in 2007. Methods IgM and IgG of the paired serums were detected by ELISA. The collected herpes were inoculated into MRC-5 cells to get VZV isolations and identify them by VZV standard serums. A 268bp fragment was amplified by specific PCR primers. Results Among the five paired serums, two of the acute phase serums were VZV IgM positive, one was VZV IgG positive, and two were equivocal for anti-VZV IgG. All of the convalescent serums were VZV IgG positive, and four of them were 4 times higher in antibody titer than those of acute phase serums. Viruses were isolated from all of the 5 herpes, and then identified by VZV standard serum. The electrophoresis result of PCR products showed the single specific strap. Conclusions This outbreak was caused by VZV virus. 47.4% of the patients were inoculated in postnatal or domestic VZV vaccines in China 3 years after delivery. The effectiveness of VZV vaccine has been certified. It is necessary to consider the immunization strategy of VZV vaccine.

关 键 词：水痘-带状疱疹病毒 爆发 免疫策略 

分 类 号：R373.12[医药卫生—病原生物学]