罗格列酮对胰岛素抵抗人肝细胞Angpil3和LXRα基因表达的影响  

Effect of Rosiglitazone on the Gene Expression of Angpil3 and LXRα of Insulin-resistant Human Liver Cells

作　　者：黄家利[1] 王继红[1] 程梅[1] 曾建涛[1] 

机构地区：[1]重庆医科大学基础医学院生物化学与分子生物学教研室,重庆400016

出　　处：《生物技术通报》2009年第5期101-103,108,共4页Biotechnology Bulletin

基　　金：重庆医科大学创新基金(CX200528)

摘　　要：研究罗格列酮对胰岛素抵抗人肝L02细胞Angptl3基因及与脂代谢相关的LXRα基因的影响。采用高胰岛素诱导法建立胰岛素抵抗模型(IR-L02),分别加入含有和不含罗格列酮的不同葡萄糖浓度培养液培养,用葡萄糖氧化酶-过氧化物酶法(GOD-POD法)检测培养液残存葡萄糖量,并用RT-PCR方法检测基因Angptl3、LXRα mRNA表达水平的变化。结果表明相同葡萄糖浓度下罗格列酮作用组(IR-R组)的培养液残存葡萄糖量比无罗格列酮作用组(IR组)减少;IR-R组的Angptl3、LXRα mRNA表达水平较IR组显著升高(P<0.05)。The research was to investigate the effect of rosiglitazone on gene Angptl3 and LXRα of insulin-resistant L02 cells. An insulin-resistant 1.02 cell with high concentration insulin was established. Rosiglitazone was added in the culture solution of insulin-resistant L02 cells as treated group. The content of glucose remained in the culture solution was measured by the method of glucose oxidizes peroxides（GOD-POD）. The expression of gene Angptl3 mRNA and LXRα mRNA was detected by reverse transcription-polymerase chain reaction（RT-PCR）. Results showed that the content of glucose remained in culture solution of insulin-resistant L02 cells which added rosiglitazone was lower than the control group of without rosiglitazone. The expression of genes Angptl3 and LXRα mRNA of IRR group were higher than the IR group（P 〈0.05 ）.

关 键 词：Angptl3 LXRΑ 罗格列酮胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]