两种昆虫杆状病毒诱导的细胞凋亡  

Apoptosis Induced by Two Insect Baculoviruses

作　　者：庞义[1] 代小江[1] 施先宗[1] 龙綮新[1] 

机构地区：[1]中山大学生物防治国家重点实验室

出　　处：《中山大学学报（自然科学版）》1998年第3期1-6,共6页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金

摘　　要：首次发现粉纹夜蛾多粒包埋核多角体病毒（Ｔｒｉｃｈｏｐｌｕｓｉａｎｉｍｕｌｔｉｃａｐｓｉｄｎｕｃｌｅａｒｐｏｌｙ－ｈｅｄｒｏｓｉｓｖｉｒｕｓ，ＴｎＭＮＰＶ）和中国棉铃虫单粒包埋核多角体病毒（Ｈｅｌｉｏｔｈｉｓａｒｍｉｇｅｒａｓｉｎｇｌｅｃａｐ－ｓｉｄｎｕｃｌｅａｒｐｏｌｙｈｅｄｒｏｓｉｓｖｉｒｕｓ，ＨａＳＮＰＶ）分别诱导斜纹夜蛾细胞Ｓｌ－ｚｓｕ－１和粉纹夜蛾细胞Ｔｎ－５Ｂ１－４发生典型的细胞凋亡．Ｓｌ－ｚｓｕ－１和Ｔｎ－５Ｂ１－４的细胞凋亡分别开始于病毒感染后１０ｈ和２４ｈ左右，其高峰期细胞凋亡率分别为９５％～１００％和６０％左右．约１０％的Ｔｎ－５Ｂ１－４细胞产生病毒包涵体．感染复数为５的病毒可诱导２种细胞的凋亡率达到高峰．ＴｎＭＮＰＶ和ＨａＳＮＰＶ的ＤＮＡ不能诱导细胞凋亡，只有用含芽生病毒的接种液攻击细胞才能诱导细胞凋亡．Apoptosis was observed in Spodoptera litura cells (Sl zsu 1) infected with Trichoplusia ni multicapsid nuclear polyhedrosis virus( Tn MNPV) and in Trichoplusia ni cells(Tn 5B1 4) infected with Heliothis armigera singlecapsid nuclear polyhedrosis virus( Ha SNPV) for the first time. Apoptotic cell death of Sl zsu 1 cells started about 10 h after infection and progressed from 10 to 24 h.Peak mortality of Sl zsu 1 cells at 24 h after infection was approximately 95% to 100%. Apoptotic cell death of Tn 5B1 4 cells started at about 24 h after infection and peak motality of the cells at 48 h after infection was about 60%.Occlusion bodies were visible in about 10% of Tn 5B1 4 cells infected with Ha SNPV. Apoptotic response of the two cells progressed and intensified when the cells were infected by the viruses at increasing multiplicit of infection (MOI) (from 0 1 to 5), and peak motality of Sl zsu 1 and Tn 5B1 4 cells were approximately 95% and 60% respectively when the cells were infected by the viruses at a MOI of 5 or more than 5. Apoptosis was found in Sl zsu 1 and Tn 5B1 4 cells infected with budded virus(BV) containing inoculum rather than transfected with viral DNA. These results indicate that host cell factors play a role in blocking or interfering with apoptotic processes; there is host specificity in the p 35 gene suppressed apoptosis;some structure proteins of baculovirus might initiate apoptotic cell death.

关 键 词：细胞凋亡 杆状病毒 P35基因 昆虫 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治] Q965.8[农业科学—植物保护]