杆状病毒gp64基因可能是细胞凋亡诱导因子  

Is Baculovirus gp 64 Gene A Primary Factor Inducing Apoptosis of Insect Cells?

作　　者：代小江[1] 庞义[1] 农广[1] 施先宗[1] 

机构地区：[1]中山大学生物防治国家重点实验室

出　　处：《中山大学学报（自然科学版）》1998年第3期7-12,共6页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金

摘　　要：发现野生型苜宿丫纹夜蛾多粒包埋核多角体病毒（Ａｕｔｏｇｒａｐｈａｃａｌｉｆｏｒｎｉｃａｍｕｌｔｉｃａｐ－ｓｉｄｎｕｃｌｅａｒｐｏｌｙｈｅｄｒｏｓｉｓｖｉｒｕｓ，ＡｃＭＮＰＶ）诱导斜纹夜蛾（Ｓｐｏｄｏｐｔｅｒａｌｉｔｕｒａ）细胞Ｓｌ－ｚｓｕ－１发生典型的细胞凋亡．该细胞凋亡发生于病毒感染后１０～１２ｈ，在病毒感染后的２４ｈ左右的高峰期细胞凋亡率为９５％～１００％．ＡｃＭＮＰＶＤＮＡ和包埋型病毒粒子不能诱导细胞凋亡，只有芽生型病毒粒子才能诱导细胞凋亡．ＡｃＭＮＰＶｇｐ６４基因编码的ＧＰ６４膜融合蛋白不仅是位于芽生型病毒粒子囊膜上已知唯一的病毒结构蛋白，还是病毒感染时最早接触细胞的病毒因子．首次提出ＡｃＭＮＰＶｇｐ６４基因编码的ＧＰ６４膜融合蛋白可能是一种细胞凋亡诱导因子，并设计了构建ＡｃＭＮＰＶＧＰ６４膜融合蛋白异源包装病毒的实验方案以便加以证实．Apoptosis was observed in Spodoptera litura cell line Sl zsu 1 induced by Autographa californica multicapsid nuclear polyhedrosis virus ( Ac MNPV).Apoptotic cell death appeared at 10￣12 h after infection, and peak mortality was approximately 95%￣100%. The Apoptosis was found in the Sl zsu 1 cells infected with budded virions but not in the cells transfected with viral DNA or infected with polyhedrin derived virions. Ac MNPV GP64 membrane fusion protein encoded by gp 64 gene is the major envelope glycoprotein of the budded virion, and is the earliest viral factor touching cells when cells were attacked by budded virion. A hypothesis was proposed that Ac MNPV GP64 membrane fusion protein gene might be a primary apoptosis inducer according with our experimental results and literature analysis. An experimental program has been designed to prove the hypothesis.

关 键 词：杆状病毒 gp64基因 细胞凋亡 诱导因子 昆虫 

分 类 号：Q939.44[生物学—微生物学] S476.13[农业科学—农业昆虫与害虫防治]