链脲佐菌素诱发糖尿病性肾病大鼠模型的特点  

Characteristics of a rat model of diabetic nephropathy induced by streptozotocin

作　　者：朱欣凯[1,2] 黄琳仪[1] 黄锐[1,2] 韩春光[1] 王琼[1] 刘永学[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]江西中医学院,南昌330004

出　　处：《军事医学科学院院刊》2009年第2期141-143,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：北京市自然科学基金资助项目(No.7072058)

摘　　要：目的：建立糖尿病性肾病（diabetic nephropathy，DN）大鼠模型，观察DN发生发展过程中大鼠肾脏相关功能及结构的变化特点。方法：二级Wistar雄性大鼠60只，体质量180～220g，分为2组：正常对照组和模型组各30只。模型组大鼠一次性腹腔注射链脲佐菌素（STZ，65mg／kg），正常对照组注射等量的枸橼酸缓冲溶液。在注射后第6、10、12、16和32周，分别动态监测大鼠的体质量、血糖、24h尿蛋白和肌酐、肾脏质量及病理改变等。结果：随着病程的发展，模型组大鼠持续高血糖，体质量明显降低，24h尿蛋白、尿蛋白／尿肌酐比值则逐渐升高，肾脏明显肥大，而且肾小球和肾小管病变在第10周开始出现，之后病变程度逐渐加重、病变范围不断扩大，表现出较为典型的DN变化特点，诸如肾小球系膜增厚、细胞外基质增生及肾小管间质损伤等。结论：STZ可成功诱导大鼠持续性高糖血症，后者的进一步发展，又可引起大鼠肾脏功能受损和形态改变。高糖血症持续12周后，DN大鼠模型的明显特征开始显现。Objective：To establish a rat model with diabetic nephropathy （DN） and clarify its characteristics. Methods： Sixty Wistar rats were randomly divided into the control and DN model groups of thirty rats each. The DN model of rats was induced by a single dose of intraperitoneal injection of streptozotocin （STZ） at a dose of 65 mg/kg, and the control rats were injected with an equal volume of citric acid buffer. Clinical and pathological parameters of the rats,including blood glucose, body mass, protein and creatinine of 24-hour urine kidney weight and pathological changes were examined or determined at weeks 6, 10, 12, 16 and 32 after injection. Results： Compared with control rats, persistent hyperglycemia, decreasing body mass and increasing 24-hour urine protein and urine protein/urine creatinine ratio were determined with progression of the disease. In the meantime, pathological examination indicated that the kidney hypertrophy of model rats appeared early, while the changes in glomeruli and renal tubules began to appear at the 10th week, and were gradually aggravated to the stage with the typical lesions of DN in kidneys, including thickening of glomerular mesangium, extracellular matrix hyperplasia and renal tubulo-interstitial damages etc. Conclusion： STZ injection could induce a persistent hyperglycemia in rats, which then could result in the key changes of DN beginning at about 12 weeks after injection.

关 键 词：链脲佐菌素 糖尿病性肾病 大鼠 细胞外基质 高血糖症 

分 类 号：R587.1[医药卫生—内分泌]