藏药旺拉提取物对东莨菪碱致动物学习记忆障碍的改善作用及其机制研究  

Effect and mechanism of Coeloglossum viride var. bracteatum extract on scopolamine-induced deficits of learning and memory behavior of rodents

作　　者：李敏[1] 王亚芳[1] 马波[1] 刘耕陶[1] 张建军[1] 

机构地区：[1]中国医学科学院、北京协和医学院药物研究所,中草药物质基础与资源利用教育部重点实验室,北京100050

出　　处：《药学学报》2009年第5期468-472,共5页Acta Pharmaceutica Sinica

基　　金：国家重点基础研究发展计划(973计划)资助项目(2007CB507400)

摘　　要：观察藏药旺拉提取物(CE)对东莨菪碱致动物学习记忆障碍的改善作用并研究其作用机制。采用腹腔注射东莨菪碱造模,以跳台法测定小鼠的学习记忆能力,以电生理方法测定大鼠海马长时程增强(LTP)的诱导,分别以生化及放射性同位素分析法测定脑匀浆中乙酰胆碱酯酶(AChE)及胆碱乙酰转移酶(ChAT)的活性。结果表明,模型动物跳下平台的潜伏期明显缩短,LTP诱导受抑制。灌胃给予CE(5、10及20mg·kg-1)可改善模型小鼠在跳台中的表现,腹腔注射CE(5mg·kg-1)可逆转东莨菪碱对大鼠LTP诱导的抑制。此外,CE可显著增强ChAT活性,而对AChE活性无显著影响。CE可改善东莨菪碱致小鼠学习记忆障碍,其作用可能与改善海马LTP诱导及增强ChAT活性有关。The aim of the present study is to investigate the effect and mechanism of Coeloglossum viride var bracteatum extract （CE） on scopolamine-induced learning and memory deficits. Learning and memory deficits of mice were evaluated by step-down passive avoidance test. Long-term potentiation of rats was detected in the dentate gyrus of hippocampus. Brain acetylcholinesterase （ACHE） and choline acetyltransferase （CHAT） activities were also determined. The results showed that scopolamine impaired learning and memory performance and LTP induction in hippocampus. Oral administration of CE （5, 10, and 20 mg·kg^-1） significantly alleviated scopolamine-induced memory deficits measured by step-down test （P〈0.05）. CE （5 mg·kg^-1, ip） significantly reversed the inhibitory effect of scopolamine on LTP in rats. In addition, CE was found to increase the activity of ChAT in rat brain. These results suggested that CE could alleviate scopolamine-induced learning and memory deficits, which might be due to the LTP-improvement and ChAT activity enhancement.

关 键 词：旺拉 东莨菪碱 跳台 长时程增强 胆碱乙酰转移酶 乙酰胆碱酯酶 

分 类 号：R282.71[医药卫生—中药学] R965[医药卫生—中医学]