不同时间预孵化对鸡胚体外发育的影响  

EFFECT OF DIFFERENT PRE-INCUBATION ON THEIN VITRO DEVELOPMENT OF CHICKEN EMBRYOS

作　　者：吴燕燕[1] 李雪峰[1] 吴光明[1] 

机构地区：[1]华南师范大学激光生命科学研究所

出　　处：《华南师范大学学报（自然科学版）》1998年第2期44-47,共4页Journal of South China Normal University(Natural Science Edition)

基　　金：广东省自然科学基金

摘　　要：本实验将预孵化０、５６、７２ｈ的鸡胚转移到受体壳中，按常规方法再孵化２１ｄ，三组的孵化率分别为０％（０／１２），３６．５％（４／１１）和５４．５％（６／１１），移壳前预孵化０ｈ鸡胚的孵化率明显低于预孵化５６ｈ和７２ｈ的鸡胚（Ｐ＜０．０１），表明鸡胚在蛋壳内的早期发育对其以后的进一步发育至关重要．实验还比较了预孵化５６ｈ和７２ｈ实验组与对照组种蛋的失重情况．预孵化５６ｈ实验组种蛋的失重明显高于对照组（Ｐ＜０．０５），结果表明移壳后孵化过程中水分的过多散失可能是导致鸡胚死亡的一个重要原因．Chicken embryos preincubated for 0h, 56h and 72h are transferred to the surrogate eggshells and incubate for 21 days in the routine method. The hatching rates are 0%(0/12), 36.5% (4/11) and 54.5% (6/11) respectively. The hatching rates of embryos preincubated for 0h is significantly lower(P<0.01) than those preincubated for 56h and 72h. These results indicate that the early development of embryos in vitro is essential for the further development. Compared with the control group, the water loss of embryos preincubated for 56h increase significantly during the incubation (P<0.05), which shows that much loss of water is one of the important reasons resulting in embryo death.

关 键 词：鸡胚 受体壳 预孵化 体外培养 

分 类 号：S831.3[农业科学—畜牧学]