牛γ-干扰素基因的克隆及序列分析  

Cloning and Sequence Analysis of Bovine Interferon-gamma Gene

作　　者：武玉梅[1] 郝永清[1] 张爱荣[1] 史冬艳[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018

出　　处：《畜牧与饲料科学》2009年第3期18-19,21,共3页Animal Husbandry and Feed Science

基　　金：国家自然科学基金资助项目(30160183);教育部博士点基金资助项目(20070129003)

摘　　要：根据GenBank中已公布的牛γ-干扰素(BovIFN-γ)基因序列设计引物,应用一步法逆转录—聚合酶链式反应(RT-PCR)技术,以从牛外周血淋巴细胞提取的总RNA为模板,扩增出γ-干扰素成熟肽基因片断,并对其进行了克隆、测序及序列分析。结果表明,试验所设计引物可以成功用于扩增牛γ-干扰素成熟肽基因片段,所扩增序列与GenBank中已有序列同源性达99.77%。Based on the published nucleotide sequence of bovine interferon-gamma （BovIFN-γ） gene, a pair of RT-PCR primers were designed and synthesized. The mature peptide fragments of γ-interferon gene was amplified using reverse transcription polymerase chain reaction （RT-PCR）from total RNA of bovine peripheral blood lymphocytes, and then carried out cloning, sequencing and sequence analysis. The results showed that the mature peptide of BovIFN-γ gene can be successfully amplified by primers designed. Additionally, the homology between the amplified gene fragment sequence and the relevant sequence in GenBank can reach up to 99.77 %.

关 键 词：牛γ-干扰素 RT-PCR法 克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]