KAP6基因家族成员在胚胎期山羊皮肤中的表达  

Expression of KAP6 Gene Family on the Skin of Fetal Goat

作　　者：张俊霞[1] 王利[2] 尹俊[3] 李金泉[4] 张燕军[4] 

机构地区：[1]呼和浩特职业学院,内蒙古呼和浩特010051 [2]内蒙古医学院,内蒙古呼和浩特010059 [3]内蒙古农业大学生物工程学院,内蒙古呼和浩特010018 [4]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018

出　　处：《畜牧与饲料科学》2009年第3期20-21,27,共3页Animal Husbandry and Feed Science

基　　金：国家自然科学基金地区联合资助项目(30660122);内蒙古自然科学基金项目(200308020407);内蒙古自然科学基金重大项目(200408020401)

摘　　要：为研究KAP6家族成员在内蒙古阿尔巴斯白绒山羊胚胎期皮肤毛囊中的表达,在体外合成用地高辛标记的阿尔巴斯白绒山羊KAP6基因家族成员的cRNA探针,通过原位杂交法在山羊皮肤组织切片上进行定位,检测KAP6 mRNA在绒山羊胚胎期皮肤组织中的表达情况。结果发现,KAP6 mRNA在初级毛囊和次级毛囊的皮质层有强烈的表达信号,表明KAP6是绒山羊毛和绒皮质蛋白的组成成分,是皮质层中起角质化作用的重要蛋白质,直接参与了绒毛和粗毛的合成,对毛发的理化性质有重要作用。The aim of this research was to study the expression of KAP6 gene family on the skins follicle of fetal inner mongolia arbas white cashmere goat. In the present study, the gigoxin （DIG）labeled cRNA of arbas white cashmere goat KAP6 gene family was synthesized in vitro and was used to localize in the skin slice of fetal goat by chromosomal in situ hybridization technique, so as to detect KAP6 mRNA in the skin tissues of cashmere goat during embryonic period. The results showed that a strong signal in the cortex layer of both primary and secondary hair follicles was expressed. Therefore, KAP6 is the component of wool and cortex protein of cashmere and an important protein, which play a role of keratinization in cortical layer, directly involved in the synthesis of cashmere and coarse wool and affected the physico-ehemieal property of pelage.

关 键 词：绒山羊 角蛋白关联蛋白 毛囊 原位杂交 

分 类 号：Q786[生物学—分子生物学]