单叶细辛对寒饮射肺证模型大鼠肺通气功能相关指标的影响  

Effects of Single Leaf Asarum on Pulmonary Ventilation Function in Rats with Experimental Syndrome of Cold Fluid Shooting Lung

作　　者：明海霞[1] 黄世佐[1] 刘喜平[1] 李海龙[1] 金戈[1] 李杨[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000

出　　处：《中国中医药信息杂志》2009年第5期23-25,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：甘肃省教育厅基金(02B4-04)

摘　　要：目的复制寒饮射肺证动物模型,观察单叶细辛对该模型大鼠肺通气功能相关指标的影响。方法将30只Wistar大鼠随机分为单叶细辛组(单叶细辛水提液灌胃1mL/kg)、辽细辛组(辽细辛水提液灌胃1mL/kg)、盐水组(0.9%NaCl溶液灌胃1mL/kg)。各组给药均为每日1次,连续6d。在给药第6日灌胃后用7℃冷生理盐水做整肺灌洗,灌洗量2.5mL/kg,保留30s后抽出,5min后重复灌洗1次,连续5次,造成寒饮射肺模型,合并5次灌洗液备用。比较肺灌洗前后各组血栓素B2(TXB2)、内皮素(ET)、灌洗液磷脂与蛋白质含量的变化。结果整肺灌洗后盐水组TXB2、ET、蛋白质含量均明显升高,而磷脂含量较低。单叶细辛组、辽细辛组TXB2、ET、蛋白质含量较低而磷脂含量较高,与盐水组比较差异有统计学意义(P<0.05),而单叶细辛组和辽细辛组比较差异无统计学意义。结论单叶细辛可抑制寒饮射肺证大鼠TXB2、ET的产生,减少灌洗液中蛋白质含量,有效改善肺通气。Objective To study the effects of single leaf Asarum on pulmonary ventilation in experimental rats with syndrome of cold fluid shooting lung. Methods Wistar rats were randomly divided into three groups including singe leaf Asarum group [fed with water extracts of singe leaf Asarum 1 mL/（kg · d）], singe leaf Asarumi Miq group [fed with water extracts of singe leaf Asarumi Miq 1 mL/（kg · d）] and normal saline group [fed with 0.9% normal saline 1 mL/（kg · d）]. After fed continually for 6 days, whole lung of rats were washed with normal saline [7 ℃, 2.5 mL/（kg · d）] and then were perfused for 30 seconds. This treatment was repeated 5 times every 5 minutes to make the model of cold fluid shooting lung. The fluid of perfusion was collected and detected. The changes of TXB2, ET, protein and phospholipids of the fluid were measured before and aider lavage. Results TXB2, ET and protein in the perfusing fluid ascended obviously, while phospholipids were low in normal saline group. TXB2 and ET in singe leaf Asarum group and single leaf Asarumi Miq group were less than that in normal saline group （P 〈0.05）, but no statistical significance was found between single leaf Asarum group and single leaf Asarumi Miq group. Conclusions Single leaf Asarum can reduce TXB2, ET and protein in perfusing fluid enhance pulmonary ventilation in experimental rats with syndrome of cold fluid shooting lung.

关 键 词：单叶细辛 辽细辛 血栓素B 内皮素 磷脂 动物模型 

分 类 号：R285.5[医药卫生—中药学]