复方鳖甲软肝片抑制大鼠酒精性肝纤维化作用机制研究  

Study on Mechanism of Compound Biejiaruangan Troche in Inhibiting Alcohol Hepatic Fibrosis in Rats

作　　者：李丰衣[1] 孙劲晖[2] 田德禄[2] 祝世功[3] 潘慧[3] 刘霞[4] 王庆宝[5] 

机构地区：[1]中国人民解放军第302医院,北京100039 [2]北京中医药大学东直门医院,北京100700 [3]北京大学医学部,北京100083 [4]卫生部中日友好医院,北京100029 [5]泰山医学院,山东泰安271016

出　　处：《中国中医药信息杂志》2009年第5期26-28,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：山东省自然科学基金青年基金(Q2005C04)

摘　　要：目的探讨复方鳖甲软肝片对实验性大鼠酒精性肝纤维化的抑制作用及机制。方法40只Wistar雄性大鼠随机分为正常组、模型组、中药(复方鳖甲软肝片)治疗组和西药(安珐特)治疗组,以"酒精-吡唑-玉米油"混合液灌胃并饲以高脂饮食16周制备大鼠酒精性肝纤维化模型,给药4周后检测肝纤维化指标、肝组织病理学及肝组织中转化生长因子-β1(TGF-β1)蛋白表达变化。结果酒精性肝纤维化模型大鼠肝功能显著异常,肝纤维化明显;经4周治疗,与模型组比较,中药组及西药组血清中透明质酸(HA)含量显著下降(P<0.01),且中药组能显著下调大鼠肝组织中TGF-β1蛋白含量。结论复方鳖甲软肝片能够有效阻止和逆转酒精性肝纤维化的进程。Objective To probe into the inhibiting action of Compound Biejiarangan Troche on alcohol hepatic fibrosis in rats. Methods Forty Wistar male rats were divided into control, model, Compound Biejiarangan Troche and Anfate treatment groups. Alcohol hepatic fibrosis model was established by intragastrical perfusion with mixture of ＂alcohol-Pyrazole-coil oil＂ and high-fat diet for sixteen weeks. After four weeks＇ intervention, changes of hepatic fibrosis index, liver tissue pathology and expression of TGF-β1 in liver tissue were observed. Results The hepatic function of model rats were changed and abnormal, with obvious fibrosis in hepatic histopathology. After intervention for four weeks, HA decreased （P〈0.01） in serum of Compound Biejiarangan Troche group and Anfate group compared with that of model group, and expression of TGF-β1 decreased in Compound Biejiarangan Troche group compared with Anfate group. Conclusion Compound Biejiarangan Troche has effect of inhibiting and reversing alcohol hepatic fibrosis in rats.

关 键 词：复方鳖甲软肝片 酒精性肝纤维化 转化生长因子-Β1 大鼠 

分 类 号：R285.5[医药卫生—中药学]