加味补肝汤对糖尿病大鼠坐骨神经中蛋白激酶Cβ亚型表达的影响  

Influence of Jiawei Bugan Decoction on PKC-βⅡ Expression of Sciatic Nerve in Experimental Diabetic Rats

作　　者：傅擎宇[1] 黄娟[1] 陈泽奇[1] 熊丽丽[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008

出　　处：《中国中医药信息杂志》2009年第5期34-36,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：湖南省卫生厅中医药科研经费资助(6202-38);湖南省重点学科建设经费资助[湘教通(2001)179]

摘　　要：目的观察加味补肝汤对糖尿病大鼠坐骨神经中蛋白激酶Cβ亚型(PKC-βⅡ)mRNA表达的影响,探讨其对实验性糖尿病大鼠周围神经病变的防治作用。方法65只大鼠适应性喂养1周后,随机取20只为正常组,45只造模,以链脲佐菌素诱发糖尿病模型,将造模成功后的40只大鼠随机分为模型组、加味补肝汤组。分别于治疗后4、8周处死大鼠,用反转录-聚合酶链反应(RT-PCR)技术测定坐骨神经中PKC-βⅡmRNA的表达。结果治疗后4、8周模型组大鼠PKC-βⅡmRNA表达明显高于正常对照组(P<0.05),加味补肝汤组PKC-βⅡmRNA表达明显低于同期模型组(P<0.05)。结论加味补肝汤对实验性糖尿病大鼠周围神经病变有一定的防治作用。Objective To observe the effect of Jiawei Bugan decoction on PKC-βⅡ expression of sciatic nerve in experimental diabetic rat and explore its preventive and therapeutic effects on peripheral neuropathy in experimental diabetic rats. Methods In 65 rats involved, 45 rats were made as model and 20 were used as control. The diabetic rat model was established by streptozotocin （STZ）. Forty model rats were randomly divided into model group and Jiawei Bugan decoction group. The rats were killed on 4th or 8th week from the beginning of treatment respectively, and PKC-βⅡ mRNA of sciatic nerve was detected by reverse-transcriptase polymerase chain reaction （RT-PCR）. Results PKC-βⅡ expression of the model group on 4th or 8th week was higher than that of the normal control group （P 〈0.05）, while that of Jiawei Bugan decoction group were markedly lower than the model group （P 〈0.05）. Conclusion Jiawei Bugan decoction has preventive and therapeutic effect on peripheral neuropathy in experimental diabetic rats.

关 键 词：加味补肝汤 糖尿病 坐骨神经 蛋白激酶C β 亚型 大鼠 

分 类 号：R289.11[医药卫生—方剂学]