左旋四氢巴马汀对急性全脑缺血-再灌注大鼠肝组织血红素氧合酶-1与内源性一氧化碳的影响  

Effects of L-THP on Hemeoxygenase-1 Carbon Monoxide during Global Cerebral Ischemia-reperfusion in Rats

作　　者：屠恩远[1] 曾爱英[1] 杨光田[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科,武汉430030

出　　处：《医药导报》2009年第5期568-571,共4页Herald of Medicine

基　　金：湖北省科技攻关计划资助项目(基金编号:2003AA301C51)

摘　　要：目的考察左旋四氢巴马汀在大鼠急性全脑缺血-再灌注损伤时对肝组织血红素氧合酶-1(HO-1)和内源性一氧化碳(CO)的影响。方法采用四血管阻塞法建立大鼠急性全脑缺血-再灌注模型,分别检测假手术组(S组,仅行假手术)、脑缺血-再灌注组(I组,制备全脑缺血-再灌注模型,0.9%氯化钠注射液每隔6h腹腔注射1次)和左旋四氢巴马汀治疗组(R组,制备全脑缺血-再灌注模型,左旋四氢巴马汀20mg·kg-1每隔6h腹腔注射1次),分别于脑缺血-再灌注后2,6,12,24,48h处死大鼠6只,检测肝组织HO-1活性和血液中碳氧血红蛋白含量。结果左旋四氢巴马汀可在2,6,12,24h提高CO及HO-1水平(均P<0.05),且在12hCO及HO-1的水平达到最高峰(均P<0.05)。结论左旋四氢巴马汀可通过升高HO-1活性使CO增加,从而减轻脑缺血-再灌注时肝组织炎症损伤,对肝组织起保护作用。Objective To investigate the effects of L-THP on hemeoxygenase-1 （ HO-1 ） in liver and endogenous carbon monoxide（CO） during global isehemia-reperfusion in rats. Methods Cerebral ischemia-repedusion model was produced in SD rats. Among three groups： sham group（ S）, isehemia-reperfusion group（I） and L-THP group（ R）, HO-1 activity in liver and COhb level in blood were evaluated, respectively at 2, 6, 12, 24 and 48 hour during global cerebral ischemia-reperfusion. Results HO-1 activity, COhb content in group R were elevated, respectively at 2,6,12,24 hour （P 〈 0.05 ）, compared with group S and I, and which reached the highest level at 12 hour （P 〈 0.05）. Conclusion L-THP could increase COhb by elevating the activity of HO-1, attenuate liver injury during global cerebral ischemia-reperfusion, and protect liver.

关 键 词：四氢巴马汀 左旋 损伤 缺血-再灌注 损伤 肝 一氧化碳 血红素氧合酶 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]