灵芪胶囊大鼠含药血清对人肺腺癌细胞A549凋亡相关基因C-myc与Survivin表达的影响  

Effects of Lingqi Contained Serum on Apoptosis Related Genes C-myc and Survivin in Human Pulmonary Adenocarcinoma 549 Cells

作　　者：刚宏林[1] 杨东光[2] 苏云明[3] 

机构地区：[1]哈尔滨市食品药品检验检测中心,150525 [2]哈尔滨医科大学附属第一医院血液科,150001 [3]黑龙江中医药大学药理教研室,哈尔滨150040

出　　处：《医药导报》2009年第5期574-577,共4页Herald of Medicine

摘　　要：目的观察灵芪胶囊(LQC)含药血清在体外对人肺腺癌A549细胞增殖及凋亡相关基因表达的影响。方法采用MTT比色法测定LQC含药血清对体外处理A549细胞株24,48,72,96h的增殖抑制率。采用RT-PCR检测A549细胞株C-myc和Survivin相关基因mRNA的表达。Wright-Giemsa染色方法观察灵芪胶囊含药血清对肿瘤细胞形态的影响。结果不同浓度LQC含药血清对A549细胞增殖具有抑制作用,抑制作用均与浓度呈正相关。RT-PCR结果显示,Survivin基因的mRNA表达水平明显下降,而C-myc基因的mRNA表达水平显著增强。同时亦使肿瘤细胞的形态结构发生改变。结论LQC含药血清对A549细胞株增殖具有抑制作用,可能与调控相关凋亡基因的表达有关,这为LQC产生抗肿瘤生物学效应提供了理论依据。Objective To investigate the effect of LQC-contained serum on apoptosis related genes expression and proliferation of human pulmonary adenocarcinoma A549 cells in vitro. Methods MTT assay was used to detect the inhibition rate of A549 cells treated with LQC-contained serum in 24, 48, 72 and 96 hours. And RT-PCR was used to measure the mRNA expression of apoptosis related genes C-myc and survivin on A549 cells. The cells morphological changes by LQC-contained serum were observed with Wright-Giemsa staining. Results The LQC-contained serum in various concentrations inhibited proliferation of A549 cells directly in a dose-dependent way. The results by RT-PCR showed that LQC- contained serum down- regulated expression of survivin gene. In contrast, it up-regulated expression of C-myc gene. LQC-contained serum also changed the morphological structure of A549 cells. Conclusion LQC-contained serum has a strong inhibitory effect on proliferation of A549 cells, and the mechanism may be related with regulation of apoptosis related genes, which may provide the strong proof for anti-tumor effects of LQC.

关 键 词：灵芪胶囊 血清药理学 A549细胞 凋亡相关基因 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]