不同遗传背景及增龄对大鼠高血压胰岛素抵抗相关基因表达谱的影响  

Effect of aging and different genetic background on insulin resistance related genes expression in rats

作　　者：田红燕[1] 万红梅[1] 马爱群[1] 韩克[1] 王亭忠[1] 胡志[1] 席雨涛[1] 范粉灵[1] 

机构地区：[1]西安交通大学第一附属医院心内科,陕西西安710061

出　　处：《第四军医大学学报》2009年第9期815-818,共4页Journal of the Fourth Military Medical University

基　　金：美国中华医学基金会项目(2002-2007)

摘　　要：目的:探讨不同遗传背景以及增龄对大鼠高血压胰岛素抵抗相关基因表达谱的影响,筛选高血压胰岛素抵抗相关基因.方法:以自发性高血压(SHR)大鼠和Wistar-Kyoto(WKY)大鼠为动物模型,用S4000点基因表达谱芯片分别检测不同遗传背景(14wk SHR大鼠和14wk WKY大鼠)的大鼠肾组织差异表达基因、增龄(SHR大鼠从5wk增龄至14wk)对SHR大鼠肾组织基因表达谱的影响,并从中随机选取2个差异表达基因,用RT-PCR方法进行验证.结果:①SHR大鼠增龄后肾组织中差异表达基因209个;14wk SHR大鼠与14wk WKY大鼠相比较肾组织差异表达基因有166个;②根据SHR大鼠增龄及不同遗传背景大鼠肾组织2种基因表达谱芯片检测结果显示,两者共同差异表达基因11个,其中免疫相关基因2个、代谢相关基因4个、其他类型基因5个.结论:代谢、免疫相关基因表达差异可能在高血压胰岛素抵抗发病中起重要作用.AIM： To explore the effect of aging and different genetic background on the insulin resistance related genes expression in rats and to screen the closely relative genes of the hypertension with insulin resistance. METHODS： Spontaneously hypertensive rats（SHR） and Wistar-Kyoto （WKY） rats were used as models and S4000 point gene-chip was used to detect the gene differential expression of kidney tissues in rats with different genetic back- ground（ ld wk SHR and 14 wk WKY）. The effect of aging（5 wk to 14 wk SHR） on the gene expression profile was also analyzed. RESULTS： A total of 209 differential genes expression in kidney tissues were found in rats with the different ages （5 wk SHR and 14 wk SHR）, and 166 differential genes expressions were found in rats with different genetic background （ 14 wk SHR and 14 wk WKY）. Results of gene-chip detection showed that there were 11 common differential expression genes in the two groups, including 2 immunity related genes and 4 energy metabolism related genes.CONCLUSION： The genes differential expression related to metabolism and immunity may play an important role in the onset of hypertension and insulin resistance.

关 键 词：高血压 基因芯片 胰岛素抵抗 

分 类 号：R544.1[医药卫生—心血管疾病]