大花蕙兰茎尖组织培养与植株再生研究  

Tissue culture and plant regeneration of shoot tip of Cymbidium grandiflorium

作　　者：徐永清[1] 胡国富[2] 李凤兰[2] 刘荣梅[2] 胡宝忠[2] 

机构地区：[1]东北农业大学成栋学院,哈尔滨150030 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2009年第4期60-64,共5页Journal of Northeast Agricultural University

基　　金：东北农业大学科学研究基金

摘　　要：文章以大花蕙兰为材料,研究其茎尖组织培养再生体系。结果表明,最佳原球茎诱导培养基为VW+0.8mg·L-16-BA+0.2mg·L-1NAA+20g·L-1蔗糖+0.05g·L-1AC,pH5.2。原球茎体分化丛生芽经壮苗生根后可进行移栽,最佳移栽基质为树皮块和水苔藓。The shoot tip of Cymbidium grandiflorium was used as explants to study the tissue culture and regeneration system. The results showed that the optimal medium for protocorm induction was VW ＋ 0.8 mg·L^-1 6-BA ＋ 0.2 mg·L^-1 NAA ＋ 0.05 g·L^-1 AC with 20 g·L^-1 sucrose, and pH 5.2. The rooted plantlets derived from protocorm could be transplanted. The optimal transplant mediums were pieces of cortex and lichen.

关 键 词：大花蕙兰 组织培养 再生体系 

分 类 号：Q949.95[生物学—植物学] S682.31[农业科学—观赏园艺]