云南4个马品种的随机扩增多态DNA(RAPD)分析  

GENETIC DIVERSITY DETECTED BY RAPD IN FOUR YUNNAN HORSE BREEDS

作　　者：史宪伟[1,2] 陈永久[1,2] 刘爱华[1,2] 张亚平[1,2] 

机构地区：[1]中国科学院昆明动物研究所细胞与分子进化开放研究实验室 [2]云南农业大学动物科技学院

出　　处：《畜牧兽医学报》1998年第3期193-203,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：云南省科委基金;国家杰出青年科学基金;国家科委高技术司基础研究专项基金

摘　　要：本文利用随机扩增多态ＤＮＡ技术研究了云南４个马品种４８个个体的遗传变异和系统发育关系。在所使用的２５个引物中，有２２个引物扩增出多态谱带。共检测到２１６条扩增片段，其中７４条出现变异。用Ｓｈａｎｎｏｎ指数计算了４个品种的遗传变异及其遗传变异在群体内和群体间的分布。用Ｎｅｉ的片段共享度公式计算了４８个个体的遗传距离，用ＵＰＧＭＡ聚类法构建了系统发育树状图。结果表明，腾冲马和新丽江马品种内的遗传变异显著高于文山马和乌蒙马，遗传变异６８．０６％存在于群体内。系统聚类图中品种内大多数个体聚在一起，文山马和乌蒙马聚成一个大类，腾冲马和新丽江马又聚成一个大类，说明文山马和乌蒙马以及腾冲马和新丽江马有较近的亲缘关系。In this study,random amplified polymorphic DNA(RAPD) analysis was used to estimate genetic diversity and relationship in 48 indivuduls which belong to four Yunnan horse breeds:Wenshan,Tengchong,Xin lijiang and Wumeng.25 primers were used,among which 22 generated polymorphic loci.A total of 216 bands were scored,of which 74(34.3%)were polymorphic.Shannon index was used to estimate the genetic diversity and the proportion of genetic variation within and between population.The genetic distance matrix was obtained by proportion of shared fragments.Dendrogram was generated by UPGMA.The results indicated that the genetic diversity of Tengchong and Xin lijiang was significantly higher than that of Wenshan and Wumeng.About sixty nine percert variation was detected within population.Most indivuduls in the same breeds were clustered together.Tengchong breed is closely related to Xin lijiang breed,and Wenshan breed is closely related to Wumeng breed.

关 键 词：云南马 遗传结构 RAPD 马 品种 

分 类 号：S821.2[农业科学—畜牧学]