牦牛肝片吸虫分泌排泄抗原的生化特性和免疫印迹分析  

BIOCHEMICAL CHARACTER OF EXCRETORY/SECRETORY ANTIGENS OF YAK FASCIOLA HEPATICA AND ANALYZING BY WESTERN BLOTTING

作　　者：徐恒[1] 吴峰[1] 沈斌[1] 刘世贵[1] 张西玉 

机构地区：[1]四川大学草原生物工程国家实验室 [2]乐山高等师范专科学校

出　　处：《畜牧兽医学报》1998年第3期247-253,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"八五"攻关项目

摘　　要：肝片吸虫的分泌排泄抗原（ＥＳ抗原）在诊断及诱导机体产生抗体方面具有重要作用。本文对寄生于牦牛的肝片吸虫ＥＳ抗原的蛋白质组成及其生化特性进行了分析。ＳＤＳ聚丙烯酰胺凝胶电泳显示ＥＳ抗原共有９条带，主要由１２－２６ＫＤ的小分子量蛋白质组成；糖蛋白及脂蛋白染色后发现ＥＳ抗原中糖蛋白极少，而含有较多的脂蛋白；等电聚焦电泳结果显示ＥＳ抗原有２２条带，几乎全部是酸性蛋白，ｐＩ主要集中在４．２５～５．２５范围内；双向电泳共检出３０个多肽，主要分布在ｐＩ４．５～７．０之间；免疫印迹分析结果表明：ＥＳ抗原中分子量为１６．２ＫＤ～１８．６ＫＤ的蛋白质是主要的抗原成分，其中１７．２ＫＤ多肽具有最强的免疫原性。The excretory/secretory antigens of Fasciola hepatica have been demonstrated to be main antigens with value for both diagnosis and inducing resistance. This paper analyzed protein composition and its biochemical character of ES antigens of Fasciola hepatica parasi ting on yak.SDS PAGE showed that ES antigens have 9 bands which mainly have smaller molecular weight of 12 26KD;Gluco and lipo protein stain showed little glucoproteins and larger lipoproteins were observed in ES antigens;IEF indicated that ES antigens had 22 bands,mainly consisted of acidic proteins(pI 4 2 5.25);Two dimenstion electrophoresis of ES showed 30 bands in pI 4.5～7.0;Immunoblotting indicated that proteins of MW 16.2 18 6KD we the major antigens,and the immunogenecity of 17.2KD was the strongest.

关 键 词：牛病 肝片吸虫 分泌排泄抗原 免疫印迹 牦牛 

分 类 号：S858.235.9[农业科学—临床兽医学] S852.43[农业科学—兽医学]