酿酒酵母和糖化酵母核倍性融合株的生长与生理特性研究  

Growth and physiological properties of different ploided fusants of Saccharomyces cerevisiae and Saccharomyces diastaticus.

作　　者：陈国潮 曾云中 

机构地区：[1]浙江农业大学土地科学和应用化学系 [2]杭州大学生物系

出　　处：《浙江农业大学学报》1998年第3期263-267,共5页

基　　金：国家自然科学基金

摘　　要：出发菌株是通过原生质体融合而获得的酿酒酵母（Ｓ．ｃｅｒｅｖｉｓｉａｅ）菌株ＨＵ－ＴＹ－１Ａ与糖化酵母（Ｓ．ｄｉａｓｔａｔｉｃｕｓ）菌株５２０６－１Ｂ的不同核倍性融合株．经对其生长速率、生物量、耐渗性、耐酒精能力以及发酵力等的测定，表明均不同于原双亲株ＨＵ－ＴＹ－１Ａ和５２０６－１Ｂ；同时发现两株性状优异的四倍体菌株：４ＡＢ２－６０和４ＢＡ１－３．在３０℃培养时，其生长速率和生物量略高于亲株或与之相当，４０℃时明显高于双亲株，耐高渗、耐酒精能力高于５２０６－１Ｂ，与ＨＵ－ＴＹ－１Ａ相当；３０℃淀粉发酵与５２０６－１Ｂ接近，４０℃时则优于５２０６－１Ｂ；３０℃葡萄糖发酵，４ＢＡ１－３低于５２０６－１Ｂ，高于ＨＵ－ＴＹ－１Ａ，４ＡＢ２－６０则优于双亲株，４０℃时，４ＡＢ２－６０和４ＢＡ１－３均优于双亲株．同时我们还发现不同倍性的同株融合株之间存在一定的基因表达剂量效应．Tested strains are different ploided fusants of S. cerevisiae strain HU TY 1A and S. diastaticus strain 5206 1B by means of protoplast fusion.Comparative studies of growth rate and biomass, osmotic pressure and alcohol tolerance, and fermentation ability between the fusants and the parental strains showed that the fusants were quitely different from parental strains HU TY 1A and 5206 1B;While two fusants,4AB 2 60 and 4BA 1 3 which were both 4 n ,were found to be prominent in physiological properties.They were both thermotolerant, resistant to high osmotic pressure, alcohol tolerant , and better at fermenting starch and glucose than their parents, especially at 40 ℃. It was also found that there existed a gene dosage effect with single parental fusants.

关 键 词：原生质体融合 融合株 酿酒酵母 糖化酵母 

分 类 号：TS261.1[轻工技术与工程—发酵工程] Q949.326.1[轻工技术与工程—食品科学与工程]