利用氢自养反硝化菌处理硝酸盐污染地下水的研究  

STUDY ON CONTAMINITATED GROUND WATER TREATMENT WITH HYDROGENOTROPHIC DENITRIFYING BACTERIA

作　　者：张彦浩[1,2] 钟佛华[1] 夏四清[1] 

机构地区：[1]同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海200092 [2]山东建筑大学市政与环境工程学院,山东济南250101

出　　处：《水处理技术》2009年第5期75-78,104,共5页Technology of Water Treatment

基　　金：国际科技合作项目(2007DFR90050);教育部新世纪优秀人才支持计划项目(NCET-05-0387);世博专项(07DZ05814)

摘　　要：讨论了氢自养反硝化菌的驯化培养方法及氢自养反硝化菌在厌氧条件下利用氢气作为电子供体还原硝酸盐的可行性,试验建立了氢自养反硝化菌生物量的定量方法,每单位OD600相当于水样中氢自养反硝化菌的生物量浓度为491.74mg·L-1;讨论了硝酸盐底物浓度对氢自养反硝化菌反硝化性能的影响,硝酸盐浓度超过150mgNO3--N·L-1时,反硝化菌的活性会受到抑制;在氢自养混合菌初始生物量25mg·L-1左右,硝酸盐浓度为100mgNO3--N·L-1以下时,反硝化时间21h可以达到硝酸盐的彻底还原。Nitrate contaminated ground water was treated with hydrogenotrophic denitrifying bacteria and the cultivation of hydrogenotrophic denitrifying bacteria, the feasibility of the denitrification with hydrogen as electron donor have been studied. The correlation of biomass and OD600 for hydrogenotrophic denitrifying bacteria was established with 491.74 mg biomass· L^-1 OD600 unit. The effect of substrate concentration on hydrogenotrophic denitrification was discussed, and the result showed that the denitrification activity was significantly inhibited at nitrate concentration more than 150mgNO3^--N·L^-1, and the nitrate could be completely reduced in 21h with initial biomass 25mg·L^-1 at the nitrate concentration lower than 100mgNO3^--N·L^-1.

关 键 词：氢自养反硝化 硝酸盐 地下水 氢气 

分 类 号：X523[环境科学与工程—环境工程]