上皮细胞钙粘蛋白与β-连环蛋白在恶性腹水鉴别诊断中的价值  

An Evaluation of E-cadherin and β-catenin in differentiation of Malignant Ascites

作　　者：李晓霞[1] 操寄望[1] 罗和生[1] 

机构地区：[1]武汉大学人民医院消化科,430064

出　　处：《临床内科杂志》2009年第5期315-317,共3页Journal of Clinical Internal Medicine

摘　　要：目的通过检测不同病因腹水中上皮细胞钙粘蛋白（E—cadherin）和β-连环蛋白（β-catenin）的含量，探讨其在鉴别良、恶性腹水性质中的意义。方法收集41例来自临床上已确诊患者的腹水标本，分为结核组（5例）、肝硬化组（11例）和恶性肿瘤组（25例）3组，采用酶联免疫吸附法（ELISA）检测E—cadherin和β—catenin的含量。结果结核性腹水E—cadherin水平为（7．57±0．48）ng／L，肝硬化腹水水平为（8．18±0．81）ng／L，恶性腹水水平为（9．35±1．84）ng／L。结核性腹水β-catenin水平为（0．76±0．13）ng／L，肝硬化腹水为（0．93±0．06）ng／L，恶性腹水为（1．67±1．16）ng／L。恶性腹水E—cadherin和β-catenin水平明显高于结核性腹水和肝硬化性腹水（P〈0．05），两者水平在结核性腹水和肝硬化腹水之间比较差异无显著性（P〉0．05）。相关分析表明E—cadherin和β—catenin的表达具有正相关性（P〈0.01，r=0．479）。结论E-cadherin和β-catenin与肿瘤的浸润和转移有关，恶性腹水中两者的水平显著增高。E—cadherin和β—catenin对良、恶性腹水鉴别诊断有重要价值。Objective Analysis the levels of E-cadherin and β-catenin in neoplastic,hepatic cirrhotic and tuberculous ascitic fluid to determine the value of E-cadherin and β-catenin for differentiating [ min1 ]. Methods E-cadherin and β-catenin levels were detected in ascitic fluid from 5 patients with tuberculous disease, 11 patients with cirrhosis, and 25 patients with neoplasm by EL ISA methods. Results The level of E-cadherin was 7.57 ± 0.48 ng/ L in tuberculous group,8.18 ± 0. 81 ng/ L in cirrhosis group,and 9.35 ± 1.84 ng/L in neoplastic group. The level of β-catenin was 0.76±0.13 ng/L in tuberculous group,0.93 ±0.06 ng/ L in cirrhotic group,and 1.67 ± 1.16 ng/L in neoplastic ascites . E-cadherin and β-catenin in patients with neoplasm were much higher than those with tuberculous disease and cirrhosis（P 〈0. 05 ）. No difference of those between hepatic cirrhosis and tuberculous peritonitis was found. There was positive correlation between the expression of E-cadherin and β-catenin （ P 〈 0. 01, r = 0.479）. Conclusion E-cadherin and β-catenin expression is involved in the incursion and metastasis of the neoplasm,and the levers of them were significantly elevated in neoplastic group. So there would be some difference between malignancy-related and no-malignancy-related ascites. The detection of E-cadherin and β-catenin levels is useful in the differential diagnosis of benign and malignant ascites.

关 键 词：恶性腹水 上皮细胞钙粘蛋白 Β-连环蛋白 

分 类 号：R446.1[医药卫生—诊断学]