血管生成素1基因转染对胃癌细胞侵袭能力的影响  

Effects of angiopoietin-1 on invasion of human gastric cancer cell line BGC-823

作　　者：杭程[1] 欧希龙[1] 陈慧娟[1] 杨柳[1] 颜芳[2] 关云艳[3] 孙为豪[4] 

机构地区：[1]东南大学附属中大医院消化病科,南京210009 [2]第二军医大学附属长海医院肿瘤内科 [3]无锡市中医院ICU [4]南京医科大学第一附属医院老年科

出　　处：《江苏医药》2009年第5期567-569,共3页Jiangsu Medical Journal

摘　　要：目的探讨人血管生成素1(Ang-1)对人胃癌细胞BGC-823基质金属蛋白酶2(MMP-2)表达情况的影响。方法利用腺病毒作为载体将已构建成功的含Ang-1基因的重组腺病毒(Ad-Ang-1)瞬时转染人胃癌细胞BGC-823为实验组,转染编码绿色荧光蛋白的复制缺陷型腺病毒重组体(Ad-GFP)为阴性对照组,未转染的正常细胞为对照组。应用RT-PCR和Western blot印迹检测三组细胞中MMP-2在mRNA和蛋白表达的情况。结果MMP-2在实验组中表达明显高于阴性对照组和空白对照组(P<0.05)。结论Ang-1能显著提高人胃癌细胞BGC-823的MMP-2的表达,提示Ang-1可能具有促进肿瘤细胞侵袭的作用。Objective To explore the effects of angiopoietin-1 on matrix metalloproteinase-2 （MMP-2） of human gastric cancer cell line BGC-823. Methods The cell line of BGC-823 was transfected with Ad-Ang-1 and Ad-GFP. The cell line of BGC-823 without transfection was taken as the control. The expression of MMP-2 mRNA was detected by reverse transcription polymerase chain reaction （RT-PCR）. The protein expression of MMP-2 was detected by Western blot. Results The expression of MMP-2 was significantly higher in Ad-Ang-1 transfected group than that in transfected group and Ad-GFP group （P〈0.05）. Conclusion Transfection of Ang-1 into human gastric cancer cell line BGC-823 can significantly increase the expression of MMP-2 suggesting that Ang-1 may promote tumor invasion.

关 键 词：血管生成素1 胃癌 基质金属蛋白质酶2 

分 类 号：R521[医药卫生—内科学]