血红蛋白在纳米孔径氧化铝膜修饰电极上的直接电化学  

Direct Electrochemistry of Hemoglobin Immobilized on Glassy Carbon Electrode Modified with Anodic Alumina Oxide Films

作　　者：武五爱[1] 郭满栋[1] 尹志芬[1] 尉景瑞[1] 李婧芳[1] 李君[1] 张宇[1] 

机构地区：[1]山西师范大学化学系,临汾041004

出　　处：《化学学报》2009年第8期781-785,共5页Acta Chimica Sinica

基　　金：山西省自然科学基金(No.20001057)资助项目

摘　　要：运用电化学包埋法成功将血红蛋白(Hb)固定于纳米孔阳极氧化铝膜(AAO)修饰的玻碳电极(GC)表面,制得Hb/PPy/AAO/GC修饰电极,并对Hb/PPy/AAO/GC修饰电极的制备条件进行了优化.研究了Hb/PPy/AAO/GC修饰电极在磷酸缓冲液(pH=6.8)中的电化学行为,探讨了血红蛋白在AAO修饰电极表面的直接电子转移机理.结果表明阳极氧化铝膜不仅保持了血红蛋白的生物活性,而且通过它的纳米尺寸效应,实现了Hb与电极之间的直接电子转移.其研究内容对生命科学和临床医学有着重要意义.A steady Hb/PPy/AAO/GC electrode was obtained by successfully immobilizing hemoglobin （Hb） on a glassy carbon （GC） electrode, which was modified using anodic alumina oxide （AAO） films. Its optimum experimental conditions were found. The electrochemistry behavior of the Hb/PPy/AAO/GC biosensor in PBS （pH=6.8） and the direct electron transfer mechanism of Hb were discussed, finding that the anodic alumina oxide films kept the biological activity of Hb. Moreover through nano-size effect of the films, the direct electron transfer between Hb and the electrode finished. The study is important to life science and clinical medicine.

关 键 词：血红蛋白 氧化铝膜 化学修饰电极 

分 类 号：Q51[生物学—生物化学]