离子束诱变筛选SG^r和ara^-双突变肌苷高产菌  

Screening of High Inosine-producing Strains with Double Mutations of SG^r and ara^- by N^+ Ion Implantation

作　　者：刘国生[1] 吴飞燕[1] 赵婷[1] 王秀强 李宗义[1,3] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]新乡拓新生化科技有限公司,河南新乡453000 [3]河南省离子束生物工程重点实验室,河南郑州450052

出　　处：《中国医药工业杂志》2009年第5期338-340,共3页Chinese Journal of Pharmaceuticals

基　　金：河南省教育厅自然科学研究计划项目(2008A180018);河南省高校青年骨干教师资助项目(2005046)

摘　　要：以枯草芽孢杆菌HSD060为出发菌株,注入不同剂量N^+离子束,定向筛选磺胺胍抗性(SG^r)或阿拉伯糖利用缺陷株(ara^-)单突变株,复筛得到产量提高显著的单突变株B13(SG^r)和B67(ara^-),肌苷的摇瓶发酵产量分别比出发菌株提高16.3%和20%。注入剂量为3×10^(15)ions/cm^2时,SG^r突变率较高:1×10^(16)ions/cm^2时,ara^-突变率较高;且发生SG^r突变的频率高于ara^-突变。再次以最适剂量分别对B13、B67菌株进行离子束诱变,筛选SG^r和ara双突变菌株,经复筛得到产量显著提高的双突变株B13-105、B13-5和B67-1,平均肌苷发酵产量比相应的单突变株分别提高23.5%、20.4%和23.1%。Different N＋ ion doses were implanted into an inosine-producing strain Bacillus subtilis HSD060 for the screening of anti-sulfaguanidine （SGr） and arabinose utilization deficiency （ara-） mutants. Two single mutants, B13 （SGr） and B67 （ara）, were obtained and their inosine yields in shake flask were increased by 16.3% and 20%, compared with the original strain. The comparatively higher mutation rate of SGr mutants occurred at implantation dose of 3×10^15 ions/cm2, while that of ara mutants at 1 × 10^16 ions/cm2. Strains B13 and B67 were treated with the optimum N＋ ion dose once again for the screening of double mutated strains with SGr and ara-. Mutants B13-105, B13-5 and B67-1 were obtained with double mutations, the inosine yields of which in shake flask were increased by 23.5 %, 20.4% and 23.1%, respectively, compared with their parent strains.

关 键 词：肌苷 枯草芽胞杆菌 N^+离子束 磺胺胍抗性 阿拉伯糖利用缺陷 

分 类 号：Q93[生物学—微生物学]