d-生物素有关物质的HPLC测定  

Determination of d-Biotin Related Substances by HPLC

作　　者：陈建辉[1,2] 潘一斌[2] 陈斌[3] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009 [2]浙江医药股份有限公司新昌制药厂,浙江新昌312500 [3]绍兴文理学院化学化工学院,浙江绍兴312000

出　　处：《中国医药工业杂志》2009年第5期368-370,共3页Chinese Journal of Pharmaceuticals

基　　金：国家科技兴贸行动专项(2006GH553302);浙江省重大科技专项(2006C11190)资助

摘　　要：建立了HPLC法测定d-生物素的有关物质。采用C_(18)色谱柱,流动相为0.05%三氟乙酸溶液-乙腈(75:25),检测波长210 nm。d-生物素在10～1000μg/ml浓度范围内线性关系良好,检测限为0.5ng。有关物质的检测限均小于1.0 ng。An HPLC method was established for the determination of related substances of d-biotin. A Ct8 column was used with the mobile phase of 0.05 % trifluoroacetic acid solution-acetonitrile （75 ： 25） at the detection wavelength of 210 nm. The calibration curve of d-biotin was linear in the concentration range of 10 - 1 000 μg/ml. The limit detection of d-biotin was 0.5 ng, as its related substances were all less than 1.0 ng.

关 键 词：D-生物素 有关物质 高效液相色谱 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]