角膜穿通伤合并海水浸泡致房水肿瘤坏死因子-α含量变化的实验研究  

Effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits

作　　者：范钦华 郭斌 曹丽 陈倩 杨艳秋 陈雨花 

机构地区：[1]解放军第八一医院眼科,江苏省南京210002

出　　处：《中华航海医学与高气压医学杂志》2009年第2期82-84,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：基金项目：南京军区医学科学技术研究“十一五”课题资助项目（06MA66）

摘　　要：目的观察爆炸致兔角膜穿通伤合并海水浸泡的房水肿瘤坏死因子-α（TNF-α）含量的变化及意义。方法成年健康灰兔10只，单支鞭炮致兔角膜爆炸伤。于角膜中周部作长3mm的全层切口。右跟为实验眼，左眼为对照眼。通过角膜切口将海水注入实验眼前房，海水持续灌注跟表30min。对照眼使用生理盐水灌注相同时间。造模后第1、2．3、5、7天采用双抗体夹心ELISA法测定房水中TNF-α含量。结果实验眼造模后第1、2、3天房水中TNF-α含量分别是190．338、156．107、117．018ng／L，与对照眼相比差异有统计学意义（P〈0．01）。其中第1天TNF-α含量最高。结论爆炸导致的兔角膜穿通伤合并海水浸泡后早期房水TNF-α含量明显高于对照眼，提示TNF-α参与这一病理改变并发挥重要作用。Objective To observe effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits. Methods 10 adult healthy grey rabbits were used for the experiment. The rabbit eye model of cornea blast injury was developed by using a single firecracker. For each rabbit, the fight eye was the experimental eye （seawater was injected into the anterior chamber through the cornea incision, simulating seawater immersion） and the left eye was the control （treated with 0. 9% sodium chloride）. Levels of aqueous humor TNF-α were detected by using double-antibody sandwich ELISA on the 1st, 2nd, 3rd, 4th, 5th, and 7th days following seawater immersion or 0. 9% sodium chloride injection. Results Following development of the model, levels of aqueous humor TNF-α in the experimental eye on the 1st, 2nd, 3rd days were 190. 338, 156. 107,117. 018 ng/L respectively, and significant differences could be seen when eomparisons were made between the experimental and control eyes. The level of aqueous humor TNF-α on the 1st day was the highest and obviously reached the peak. Conclusions Level of aqueous humor TNF-α in the experimental eye with, penetrating cornea injury combined with seawater immersion was significantly higher than that of the control eye, indicating that TNF-α was clearly involved in the pathological change and it played an important role in the process.

关 键 词：角膜穿通伤 海水浸泡 肿瘤坏死因子-Α 房水 

分 类 号：R686[医药卫生—骨科学]