芍药组培中抗外植体褐化相关问题的研究  

The Collelative Research on Browning Prevention of Paeonia lactiflora Tissue Culture

作　　者：王瑶[1] 岳桦[1] 

机构地区：[1]东北林业大学园林学院,哈尔滨150040

出　　处：《湖北农业科学》2009年第4期783-786,共4页Hubei Agricultural Sciences

摘　　要：以引种黑龙江省野生生境芍药Paeonia lactiflora为供试材料,对外植体取材部位、培养条件、抑褐剂几方面进行了褐化抑制的相关研究。结果表明,叶片接种于改良MS+2,4-D0.5mg·L-1+6-BA0.5mg·L-1+蔗糖30g·L-1+琼脂8g·L-1培养基,叶柄和茎段接种于MS+6-BA3.5mg·L-1+蔗糖30g·L-1+琼脂8g·L-1培养基;并且在两培养基中添加1g·L-1的PVP,就能够有效地抑制外植体的褐化,使褐化率降为0。This paper used wild Paeonia lactiflora which from the province of Heilongjiang as experiment material. Do some collelative research on browning prevention of explants adopted different part, cultivate condition, browing inhibitor. The resuits indicated： To use leaf as explant, on the medium, modified MS＋2,4-D 0.5 mg·L^-16-BA 0.5 mg·L^-1sucrose 30g·L^-1＋ ager 8 g·L^-1, to use leafstalk and caudex as explant, on the medium,MS＋6-BA 3.5 mg·L^-1sucrose 30 g·L^-1ager 8 g·L^-1, the two medium which swing in with 1 g·L^-1 PVP took on the best browning prohibited effect, browning rate reached to 0.

关 键 词：芍药 组织培养 褐化 

分 类 号：S682.1[农业科学—观赏园艺] Q943.1[农业科学—园艺学]