活血生肌类中药对大鼠急性骨骼肌钝挫伤后氧化应激的影响  

Effects of Chinese Medicine HUOXUESHENGJI on the Oxidative Stress in Rat Skeletal Muscles after Acute Contusion

作　　者：王茂林[1] 陈世益[1] 李云霞[1] 陈疾忤[1] 李宏云[1] 

机构地区：[1]复旦大学附属华山医院运动医学科,上海200040

出　　处：《中国运动医学杂志》2009年第3期289-292,共4页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金项目(30771044)资助

摘　　要：目的:观察活血生肌类中药对大鼠骨骼肌钝挫伤后组织修复过程中氧化应激的影响。方法:72只雄性SD大鼠随机分为中药治疗组、生理盐水组、自然愈合组,每组24只。采用打击装置造成大鼠右侧腓肠肌中段钝挫伤模型,分别于各组损伤局部注射黄芪丹参注射液、生理盐水和不作处理。另6只作为空白对照(未经任何处理)。于损伤后1、4、7和14天取大鼠腓肠肌测定丙二醛(MDA)含量和过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)活性。结果:与生理盐水组和自然愈合组相比,中药治疗组腓肠肌SOD、GSH-px活性在伤后第4、7天显著升高(P<0.05),而CAT活性无明显变化(P>0.05)。中药治疗组腓肠肌MDA含量在伤后第4、7天显著低于生理盐水组和自然愈合组(P<0.05)。结论:活血生肌类中药可明显减轻骨骼肌钝挫伤愈合过程中的氧化性损伤,并显著提高骨骼肌SOD和GSH-px的活性。Objective To investigate the effects of Chinese medicine HUOXUESHENGJI （promoting blood flow and tissue regeneration） on the oxidative stress in rat skeletal muscles after acute contusion. Methods Seventy two male SD rats were randomly and equally divided into three groups： （1） Chinese medicine HUOXUESHENGJI group （A）, （2） physiological saline group （B）, （3） natural healing group （C）, and （4） blank control group （D）. The muscle contusion was produced at the gastrocnemius by specific device;Local injection at the injured site with Chinese medicine HUOXUESHENGJI in group A; local injection with physiological saline instead of HUOXUESHENGJI in group B,and natural healing group served as control. The muscles were sampled at day 1,4,7 and 14 respectively after contusion, and then the MDA and the activities of antioxidant enzymes （CAT, SOD and GSH-px） in gastrocnemius were investigated. Results As compared with the group B and group C, SOD and GSH-px activities in group A were significantly increased after contusion （P〈0.05） ,whereas,the CAT activity remained unchanged （P〉0.05）. The concentrations of MDA in group A were lower than that in group B and group C at day 4 and day 7 after contusion （P〈 0.05）. Conclusions Chinese medicine HUOXUESHENGJI could reduce the oxidative injury and increase the antioxidant ability of SOD,GSH-px in contused skeletal muscles during the healing process.

关 键 词：骨骼肌钝挫伤 活血生肌类中药 氧化应激 

分 类 号：R285[医药卫生—中药学]