光照、温度和pH对雨生红球藻光合特性的影响  

EFFECT OF LIGHTING,TEMPERATURE AND pH ON PHOTOSYNTHETIC CHARACTERS OF HAEMATOCOCCUS PLUVIALISCG-11

作　　者：王铭[1] 李涛[1] 李爱芬[1] 徐宁[1] 张成武[1] 

机构地区：[1]暨南大学水生生物研究中心,广州510632

出　　处：《水生生物学报》2009年第3期400-405,共6页Acta Hydrobiologica Sinica

基　　金：珠海市科技重大项目(PB20041018)资助

摘　　要：采用测定光合放氧速率和荧光动力学的方法,研究分析了光照强度、温度和pH对雨生红球藻Haematococcus pluvialis CG-11绿色游动细胞阶段光合作用特性的影响。结果表明,H.pluvialis CG-11光合作用的饱和光强为109.1μmol/m2·s,最大光合放氧速率为75.9μmolO2/mg Chla·h;适宜的生长温度范围在25—30℃之间,温度在25℃时光合速率最大;pH在7.5—8.0范围内,光合效率较高,在pH为7.5时放氧速率最大,为75.5μmolO2/mg Chla·h。在实验pH条件下,H.pluvialisCG-11叶绿素荧光动力学参数呈现出相似的趋势,在6.0—7.5范围内,Fv/Fm、Fv/F0、ΦPSⅡ和ETR值随pH升高而增大,pH为7.5时达到最大值,pH超过7.5时,Fv/Fm和Fv/F0值明显下降,而ΦPSⅡ和ETR的下降趋势不明显。The effects of light intensity, temperature and pH on photosynthetic characters of Haematococcus pluvialis CG-11 were studied by measuring photosynthetic 02 evolution and fluorescence kinetics. The results showed that the saturation light intensity was 109.1 μ,ol/m^2· s of H. pluvialis CG-11, with the maximum photosynthetic oxygen evolution rate of 75.9 μmol O^2/mg Chlα· h; the range of suitable growth temperature was between 25℃ to 30℃ , the maximum photosynthetic rate was obtained at 25 ℃ ; the photosynthetic efficiency kept quite high at the pH range of 7.5--8.0,with the maximum photosynthetic oxygen evolution rate of 75.5 μmol O2/mg Chla ·h at pH value of 7.5. Under experimental conditions of pH, a similar tendency manifested in the chlorophyll fluorescence kinetics parameters for H. pluvialis, at the pH range of 7.5--8.0, the Fv/Fm , Fv/F0 , ФPS Ⅱ and ETR increased following the rising of pH, with their maximum values at pH 7.5, Fv/ Fm and Fv/F0 decreased significantly over pH 7.5, while the decreasing trend didn＇ t change obviously in ФPS Ⅱ and ETR.

关 键 词：雨生红球藻 光照强度 温度 PH 叶绿素荧光 光合放氧速率 

分 类 号：Q945[生物学—植物学]