华东地区家鸭中N1亚型禽流感病毒NA基因的遗传进化分析  

Phylogenetic Analysis of the Neuraminidase Genes of Subtype N1 Viruses in Domestic Ducks in Eastern China

作　　者：仇保丰[1] 刘武杰[1] 唐应华[1] 彭大新[1] 刘秀梵[1] 

机构地区：[1]扬州大学农业部畜禽传染病学重点开放实验室,扬州225009

出　　处：《病毒学报》2009年第2期131-136,共6页Chinese Journal of Virology

基　　金：<十一五>国家支撑计划项目(2006BAD06A01);(2006BAD06A16)

摘　　要：为了解华东地区家鸭内禽流感病毒的遗传进化情况,对2002-2006年分离自华东地区家鸭的3种主要N1亚型的禽流感病毒：2株H1N1、10株H3N1和14株H5N1,共26株病毒的NA基因进行了遗传进化分析。结果表明,华东地区家鸭中的N1亚型的禽流感病毒正处于不断进化状态中。14株H5N1禽流感病毒均在NA的茎部缺失20个氨基酸（49-68位）,而其他N1亚型的禽流感病毒的NA都未见发生此缺失。H3N1病毒可能与H1N1病毒发生了NA基因的重排,但是目前还没有直接证据表明华东地区家鸭中H5N1禽流感参与了基因重排。To examine the phylogenetic information regarding the gene pool of AIV in domestic ducks in eastern China, the NA genes of twenty-six viruses isolated during 2002-2006, including two H1N1 strains, tenH3N1 strains and fourteen H5N1 strains, which reflected the predominant N1 subtype viruses were subjected to phylogenetic analysis. The results indicated that AIVs of N1 subtype circulating in domestic ducks in eastern China were undergoing a gradual evolution. Analysis of the deduced amino acid sequences revealed that NAs from all isolated H5N1 viruses had a 20-aa deletion in the stalk region （residues 49-68）, whereas no deletion was seen in the NAs from other HA subtype viruses. The viruses of H3N1 and H1N1 might have a propensity for reassortment of NA genes, whereas no direct evidence of reassortment of NA gene was obtained in HSN1 viruses.

关 键 词：家鸭 禽流感病毒 NA基因 遗传进化分析 

分 类 号：S852.659[农业科学—基础兽医学]