HPLC法测定不同产地蜂胶中白杨素的含量  

Quantitative Analysis of Chrysin from Propolis in Different Areas by HPLC.

作　　者：王小平[1] 白吉庆[1] 林励[2] 潘建国 卢占列 

机构地区：[1]陕西中医学院,陕西咸阳712046 [2]广州中医药大学中药学院,广东广州510405 [3]广州市宝生园有限公司,广东广州510400

出　　处：《四川中医》2009年第5期55-56,共2页Journal of Sichuan of Traditional Chinese Medicine

基　　金：广州市科技计划项目(编号:2005Z-E0101)

摘　　要：目的：测定国内不同产地蜂胶中白杨素的含量。方法：采用HPLC法，Diamomil（钻石）C18（46mm×250mm，5μm）色谱柱，甲醇-0．4％的磷酸水（65：35）为流动相，流速为1．0mL／min，检测波长为2．56nm。结果：白杨素在0．00592—0．03552mg／mL范围内呈良好的线性关系（R^2=0．9999）；白杨素的含量，山东最高，为23．054mg·g^-1，其次是内蒙和河南，分别为14．170mg·g^-1，13．656mg·g^-1，甘肃最低为6．697mg·g^-1。结论：不同产地蜂胶中白杨素的含量有一定差异。Objective： To mensurate the content of Chrysin in Propolis from different Producing area in China. Methods： HPLC method was established.Diamonil C18 column was used, CH3OH- 4%H3PO4 solution （65：35） as mobile phase, at the flow rate of 1.0mL/min and the UV detection wavelength at 283nm.Results： The linear of Chrysin was 0.00592 - 0.03552mg/mL （R^2 =0.9999） . the content of Chrysin was the highest in the propolis from Shandong （ 23.054 mg· g^- 1 ） . the content of Chrysin was the higher in the propolis from neimeng, and henna respectively 14.170mg·g^-1, 13.656mg·g^-1, the content of Chrysin was the lowest in the pro polis from gansu （6.697mg·g^-1） .Conclusion： The content of Chrysin in Propolis from different Producing area is different.

关 键 词：蜂胶 白杨素 产地 HPLC 

分 类 号：R284.2[医药卫生—中药学]