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出 处:《中国药房》2009年第14期1063-1065,共3页China Pharmacy
摘 要:目的:建立以反相高效液相色谱法测定人血浆中塞曲司特浓度的方法。方法:色谱柱为Lichrospher C18,流动相为0.02mol.L-1磷酸二氢钾(含0.1%三乙胺,pH=5.0)-乙腈(70∶30),流速为1.0mL·min-1,柱温为30℃,检测波长为268nm。结果:塞曲司特血药浓度在28~5600ng·mL-1范围内线性关系良好(r=0.9995),最低检测浓度为28ng·mL-1;低、中、高3种浓度相对回收率分别为102.9%、100.4%、99.7%;日内、日间RSD均<6%。结论:本法可用于塞曲司特血药浓度的测定以及其药动学研究。OBJECTIVE: To establish a RP- HPLC method for the determination of seratrodast concentration in human plasma.METHODS: The sample was determined on Lichrospher C18 column with the mobile phase consisted of 0.02 mol·L^-1 potassium dihydrogen phosphate (containing 0.1% triethylamine, pH = 5.0) - acetonitrile (70 : 30) at a flow rate of 1.0 mL·min^-1. The column temperature was 30℃ and the detective wavelength was set at 268 nm. RESULTS: The linear range of seratrodast was 28--5 600 ng·mL^-1(r = 0.999 5) with the lowest detection concentration at 28 ng·mL^-1. The recovery rates of seratrodast at low, middle and high concentrations were 102.9%, 100.4% and 99.7%, respectively; and both intraday RSD and interday RSD were all less than 6%. CONCLUSION: The method developed in this study is applicable for the determination of plasma concentration and pharmacokinetic study of seratrodast.
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