二氯二茂钛二甲硫氨酸与DNA相互作用的电化学及紫外-可见光谱研究  

Studies on the Interaction between Titanocene Di (L-Methionine) Dichloride and DNA by Cyclic Voltametry and UV-Vis Spectrometry

作　　者：杨辉琼[1,2] 刘又年[1] 

机构地区：[1]中南大学化学化工学院,湖南长沙410083 [2]湖南工程学院化学化工系,湖南湘潭411104

出　　处：《精细化工》2009年第5期485-488,共4页Fine Chemicals

基　　金：国家自然科学基金(No.20676153,20876179)资助项目~~

摘　　要：用循环伏安法考察了二茂钛二甲硫氨酸配合物(TMC)与DNA在pH=3.0的KCl水溶液中相互作用的电化学行为。TMC在玻碳电极上分别于-412 mV和-528 mV(υs.SCE)有一个氧化峰和相应的还原峰。加入DNA后,TMC的式量电位负移5 mV,峰电流增加,电化学参数(α和ks)改变。结果表明:在该条件下,TMC与DNA之间通过静电作用,形成了一种具有电活性的复合物,且TMC与DNA之间的结合位点数n和结合常数K分别为4.36和2.74×1016。结合紫外-可见光谱法测定结果,加入DNA后TMC的紫外-可见光谱在393 nm处呈现增色效应,进一步确证了TMC与DNA之间主要以静电作用为主。The electrochemical behavior of titanocene di （ L-methionine ） dichloride （ TMC ） and deoxyribonucleic acid （DNA） at pH 3.0 was investigated by cyclic Vohametry （CV）. TMC yielded an anodic peak at -412 mV and a cathodic peak at -528 mV on the glassy carbon electrode. After the addition of DNA, the formal potential of TMC negatively shifted 5 mV, the peak current of TMC increased and the electrochemical parameters （such as charge transfer coefficient ct and standard electron transfer rate constant ks） changed. The CV results showed that the electroactive complex was formed via electrostatic effects at pH 3.0. Moreover, the binding number n of 4. 36 and an affinity constant K of 2. 74×10^16 were determined. Hyperchrornism was observed in the UV-Vis spectra of TMC in the presence of the DNA. The results obtained from UV-Vis spectrometry further confirmed that the complex of TMC and DNA was formed by electrostatic effects.

关 键 词：二氯二茂钛二甲硫氨酸 DNA 循环伏安法 紫外-可见光谱法 有机电化学与工业 

分 类 号：O657.1[理学—分析化学] O657.3[理学—化学]