一株混合O血清型猪水肿病大肠杆菌的分离与鉴定  

Isolation and Identification of a Escherichia coli strain of Mixed O serotypes in piglet

作　　者：师东方[1] 葛俊伟[1] 王明翠[1] 许亚卓[1] 李海滨[1] 单安山[1] 

机构地区：[1]东北农业大学动物医学学院/动物科技学院,黑龙江哈尔滨150030

出　　处：《中国预防兽医学报》2009年第5期397-399,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：黑龙江省"十五"重点攻关项目(GB05B501);黑龙江省博士后研究基金

摘　　要：从具有典型临床症状和病理剖检变化的病死猪肠系膜淋巴结中分离到的1株革兰阴性细菌,通过菌体形态观察,染色、生化试验、SLT-Ⅱe基因检测、O血清型鉴定、动物试验,其结果证明分离菌为O26、O139、O142混合血清型,产SLT-Ⅱe大肠埃希氏杆菌。SLT-Ⅱe基因序列与GenBank上发表的参考序列(序列号为AY368993)的同源性达100%。用粗提的毒素经腹腔注射小鼠后引起小鼠后肢麻痹、死亡和胃肠水肿。A Gram-negative bacteria was isolated from the mesenteric lymph nodes of a dead weaning piglet. The bacterial strain was identified as a SLT- IIe producing Escherichia coli with mixed O serotypes after morphological observation, biochemical identification, PCR detection of SLT-IIe gene and identification of O serotype. The sequence of SLT- IIe gene of isolated strain was identical to the published sequences in GenBank （AY368993）. Injection mice with crude SLT- IIe produced hind limb paralysis, death and gastrointestinal edema.

关 键 词：猪水肿病 大肠杆菌 混合O血清型 分离鉴定 

分 类 号：S852.5[农业科学—基础兽医学]