重庆地区流行的PRRSV的ORF5基因变异分析  

Characterization of genetic variations of ORF5 of the prevalent PRRSV in Chongqing

作　　者：赵秦[1] 王永才[1] 张森[1] 吴俊伟[1] 王孝友[2] 马跃[1] 

机构地区：[1]西南大学荣昌校区动物医学系,重庆荣昌402460 [2]重庆市养猪科学研究院,重庆荣昌402460

出　　处：《中国预防兽医学报》2009年第5期403-406,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：重庆市自然基金重点项目(CSTC,2007BA1014)

摘　　要：为了分析重庆地区流行的猪繁殖与呼吸综合征病毒(PRRSV)的ORF5基因变异情况,我们通过RT-PCR扩增采自重庆地区不同猪场高热病病猪肺组织样本中PRRSV的ORF5全序列,并对其进行生物信息学分析。结果8个病例样本中有5个成功扩增出ORF5全序列。系统进化分析表明它们与国内2006年～2007年间的分离株亲缘关系较近,而与更早的分离株和疫苗MLV株亲缘关系较远。此外,推导氨基酸序列分析表明重庆地区流行的PRRSV与以JXA1株等为代表的同期其它地区分离株比较在ORF5的2个线性抗原表位中均存在独特的变异。本研究结果不仅揭示了重庆地区流行的PRRSV ORF5基因的变异特征,而且提示在PRRSV的防控措施中ORF5基因的变异应受到高度的重视。The complete ORF5 gene of PRRSV was amplified with RT-PCR from lung samples of pigs with so called ＂swine high fever disease＂ in Chongqing. Five complete ORF5 sequences were successfully amplified from 8 case samples and sequenced. The phylogenetic analysis indicated that the prevalent PRRSV in Chongqing in 2007 had closer genetic relationship with those isolates from other areas of China than the earlier field isolates and the vaccine strain MLV. There are two characteristic mutations in two lineal antigenic epitopes when compared with those of other strains such as JXA1. This study identified the characteristic ORF5 variations of prevalent PRRSV in Chongqing and highlighted the importance of the variations of PRRSV ORF5 in controlling the disease.

关 键 词：猪繁殖与呼吸综合症病毒 变异 反转录-聚合酶链式反应 高热病 

分 类 号：S852.659.5[农业科学—基础兽医学]