Polo-样激酶1在子宫内膜异位症患者在位和异位子宫内膜中的表达  

Expression of polo-like kinase 1 in the ectopic and eutopic endometrial tissue from women with endometriosis

作　　者：唐莉[1] 黄荷凤[2] 张云霞[1] 马艳萍[1] 周彩云[2] 

机构地区：[1]云南省第一人民医院生殖遗传科,650032 [2]浙江大学医学院生殖内分泌科

出　　处：《中国妇产科临床杂志》2009年第3期207-209,234,共4页Chinese Journal of Clinical Obstetrics and Gynecology

摘　　要：目的探讨Polo-样激酶1(PLK1)在子宫内膜异位症发生发展中的作用。方法采用逆转录(RT-PCR)、蛋白免疫印迹法(Western blotting)及免疫组织化学的方法检测子宫内膜异位症患者的异位子宫内膜组织29例和在位子宫内膜组织20例及正常子宫内膜组织30例中PLK1的表达。结果PLK1蛋白在内异症组增殖期异位(2.19±0.63)和在位子宫内膜组织(1.78±0.59)上的表达量明显高于对照组子宫内膜组织(1.21±0.30)上的表达量(P<0.05)。PLK1蛋白在内异症组分泌期异位(1.15±0.332)和在位子宫内膜组织(1.00±0.33)上的表达量明显高于对照组子宫内膜组织(0.82±0.24)上的表达量(P<0.05)。内异症组增殖期和分泌期异位和在位子宫内膜组织上PLK1的表达量比较,差异均无统计学意义(P>0.05)。结论PLK1蛋白的高表达可能与子宫内膜异位症的发生发展有关。Objective To investige the possible roles of polo - like kinasel （PLK1） in the pathogenesis of endometriosis. Methods The expression of PLK1 were studied in the three group tissue： 29 ectopic and 20 eutopic endometrial tissue from women with endometriosis and 30 endometrial tissue from healthy women by Western blotting, RT - PCR and immunohistochemistry. Results The expression levels of PLK1 in the ectopic and eutopic endometrial tissue from women with endometriosis were significantly higher than that in the endometrial tissue from healthy women （P〈0.05）. No significant difference was found between the expression levels of PLK1 in the ectopic and eutopic endometrial tissue from women with endometriosis （P〉0.05）. Conclusions The higher expression of PLK1 in the ectopic and eutopic endometrial indicates that PLK1 may be involved in the pathogenesis of endometriosis.

关 键 词：子宫内膜异位症 Polo-样激酶1 子宫内膜 

分 类 号：R711.71[医药卫生—妇产科学]