CO_2气腹对大鼠子宫内膜异位症病灶MMP-2和TIMP-1蛋白表达的影响  

Effect of CO_2 pneumoperitoneum on protein expression of MMP-2 and TIMP-1 of endometriosis lesion in the rat

作　　者：陈旭[1] 黄润瀚[1] 刘海防[1] 张慰[1] 刘彦[1] 

机构地区：[1]第二军医大学附属长征医院妇产科,上海200003

出　　处：《中国妇产科临床杂志》2009年第3期214-217,共4页Chinese Journal of Clinical Obstetrics and Gynecology

摘　　要：目的评价CO2气腹及不同气腹压力对子宫内膜异位症病灶基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶抑制剂-1(TIMP-1)蛋白表达的影响。方法建立SD大鼠子宫内膜异位症模型,造模成功后随机分组:对照组、10mmHg和20mmHgCO2气腹组,气腹作用时间1h。气腹干预后1周、2周和6周检测子宫内膜异位病灶MMP-2、TIMP-1蛋白表达情况。结果与对照组比较,气腹干预后1周10mmHg和20mmHgCO2气腹组子宫内膜异位病灶MMP-2蛋白表达均显著降低(P<0.001),且10mmHg组低于20mmHg组(P<0.001),可持续至2周以后。两气腹组TIMP-1表达显著增强,且10mmHg组高于20mmHg组(P<0.001)。MMP-2/TIMP-1比例下降,异位病灶组织侵袭能力下降。随着时间变化,两组MMP-2逐渐升高,TIMP-1下降,术后6周与对照组比较,差异无统计学意义(P>0.05)。结论CO2气腹作用后早期可使子宫内膜异位灶组织MMP-2下降,TIMP-1升高,侵袭力下降,低气腹压力对子宫内膜异位病灶侵袭力的抑制作用更为明显。Objective To evaluate the effect of CO2 different pneumoperitoneum pressure on protein expression matrix metalloproteinase - 2 （MMP - 2） and tissue inhibitor of metalloproteinase - 1 （TIMP - 1） of endometriosis lesion. Methods The SD rats were divided into randomly three groups： control group, 10 mmHg and 20 mmHg CO2 pneumoperitoneum after endometriosis model being founded successfully. The pressure continued for about lb. The MMP - 2 and TIMP -1 in EMs lesion were detemined at 1w, 2w and 6w. Results Compared with the control group, the protein expression level of MMP - 2 in the two CO2 pneumoperitoneum groups were decreased significantly in post-operation one week （P（0. 001）. And the effect could be continued until two weeks after pneumoperitoneum intervention. Moreover the expression level of MMP- 2 in 10mmHg group was lower than that in 20 mmHg group pneumoperitoneum （P〈0. 001）. The protein expression of TIMP- 1 increased in CO2 groups, with the higher expression in 10mmHg group （P〈0. 001）. The expression level of MMP- 2 was increased and TIMP- 1 was decreased gradually with time after CO2 pneumoperitoneum intervention, and there were no difference compared with that of the control group six weeks post - operation （P〉0.05）. Conclusion The expression of MMP - 2 is decreased and TIMP- 1 is increased in the early period CO2 pneumoperitoneum intervention. The low CCh pneumoperitoneum pressure is superior to the high pressure group in inhibition of aggression of EMs lesion.

关 键 词：CO2气腹 气腹压力 子宫内膜异位症 细胞因子 

分 类 号：R711.71[医药卫生—妇产科学]