旋转磁场联合5-FU对SP2/0细胞超微弱发光强度的影响  

Effect of Rotary Magnetic Field (RMF) Combined with 5-Fluorouracil (5-FU) on the Ultraweak Photon Emission of SP2/0

作　　者：韦淑贞[1] 山顺林[1] 杜云翔[1] 张闯[1] 沙晓锋[1] 

机构地区：[1]解放军第82医院肿瘤中心,江苏淮安223001

出　　处：《现代生物医学进展》2009年第9期1701-1702,1690,共3页Progress in Modern Biomedicine

摘　　要：目的:研究旋转磁场(RMF)联合氟尿嘧啶(5-FU)对小鼠骨髓瘤细胞(SP2/0)超微弱发光的影响,探讨两者联合对肿瘤细胞的杀伤作用。方法:SP2/0细胞分为四组:对照组,单纯5-FU化疗组(单化组),单纯磁疗组(单磁组),磁场联合5-FU组(磁化组)。用RMF、5-FU作用于SP2/0细胞,化学发光仪进行分析。结果:对照组的化学发光强度值最低,单化组、单磁组、磁化组化学发光强度值均明显高于对照组(P均<0.001)。单磁组低于单化组及磁化组,有明显差别(P<0.001);磁化组的化学发光强度值最高,高于单化组(P<0.001)。结论:磁场和5-FU均可引起SP2/0细胞化学发光强度值的变化,以磁场联合5-FU的化学发光强度值变化最明显,提示磁场单独及联合5-FU对SP2/0细胞有明显的杀伤作用。其机理可能与旋转磁场和5-FU引起细胞自由基增高有关。Objective： To study the effect of rotary magnetic field （RMF） combined with 5-FU on the ultraweak photon emission of mouse cell line SP2/0 in vitro. Methods： SP2/0 cells were randomly divided into four groups： a control group （N）, a 5-FU group （C）, a magnetic treatment group （M） and a magnetic treatment combining 5-FU group （M＋C）. The M and M＋C groups were treated with a RMF for two hours at a time, once a day. On day 4, the C and M＋C groups were treated with 5-FU 20 μg/mL. On day 5, the intensities of ultraweak photon emission were measured. Results： The intensity ofultraweak photon emission of the group N was the lowest among the four groups （P all〈0.001）. The same parameter of the group M was lower than that of the group C and group M＋C （P〈0.001）. The intensity of ultraweak photon emission of the group M＋C was much higher than that of the group C. Conclusions： Both RMF and 5-FU can change the intensities ofultraweak photon emission of SP2/0 cells and the change of RMF combined with 5-FU group was the most obvious. The RMF alone or combined with 5-FU both can kill the SP2/0 cells. The mechanism may be related to the rise of the free radical of SP2/0 cells.

关 键 词：旋转磁场 氟尿嘧啶(5-FU) 超微弱发光 SP2/0细胞 

分 类 号：Q64[生物学—生物物理学]