细菌人工染色体载体的发展和应用  

Development and Applications of Bacterial Artificial Chromosome

作　　者：陈献伟[1,2] 王会[1,3] 雷娜[1,4] 关伟军[1] 马月辉[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]石河子大学生命科学院,新疆石河832003 [3]山西农业大学文理学院,山西太谷030801 [4]山西农业大学动物科技学院,山西太谷030801

出　　处：《现代生物医学进展》2009年第9期1745-1748,1700,共5页Progress in Modern Biomedicine

基　　金：国家"863"计划项目资助(2006AA10Z198;2007AA10Z170);国家自然科学基金(30671539)

摘　　要：细菌人工染色体(Bacterial artificial chromosome,BAC)是在大肠杆菌F质粒的基础上建成的高容量、低拷贝的质粒载体。BAC载体已广泛应用于基因组文库的构建及筛选、基因组测序、新基因的发现、图位克隆、BAC微阵列、转基因和动物品种资源保存等方面。本文综述了BAC载体的发展及其应用。The development of Bacterial Artificial Chromosome （BAC）, which is a High-capacity and low copy plasmid, is based on the well-characterized Escherichia coli F-factor. BAC is currently used in a wide array of applications including genomics construction, genomics sequencing, discovery of new genes, map-based cloning, BAC microarray, Transgene and animal species saving and so on. This paper described the key elements in the development of the BAC system and its current applications.

关 键 词：BAC载体 文库构建 基因组测序 转基因 动物品种资源保存 

分 类 号：Q782[生物学—分子生物学]