IL-2调控慢性丙型肝炎患者外周血CD4^+CD25^+调节性T细胞的抑制效应  

Suppressive activity of IL-2 modulation to CD4^+CD25^+ Treg cells in patients with chronic hepatitis C

作　　者：杨江华[1] 贺蕾[2] 盛浩宇[1] 喻艳林[1] 苏川[2] 

机构地区：[1]皖南医学院弋矶山医院感染科,芜湖市241001 [2]南京医科大学病原生物学系

出　　处：《中华实验和临床感染病杂志（电子版）》2009年第2期13-15,共3页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：安徽省自然科学基金(070413111);国家自然科学基金青年基金(30700694)

摘　　要：目的探讨慢性丙型肝炎患者外周血中HCV特异性CD4+CD25+调节性T细胞的抑制活性以及外源性IL-2对CD4+CD25+Treg细胞抑制效应的影响。方法采用免疫磁珠分选18例慢性丙型肝炎患者与15例正常人外周血中CD4+CD25+T细胞与CD4+CD25-T细胞,在此两种细胞共同培养体系中加入不同浓度外源性IL-2和IL-4,检测CD4+CD25+T细胞的抑制能力以及对HCV特异性CD4+T细胞的影响。结果与正常对照相比,慢性丙型肝炎患者的CD4+CD25+Treg细胞的抑制增殖活性高(P=0.034);CD4+CD25+Treg细胞显著抑制HCV特异性CD4+T细胞的增殖;当IL-2浓度为2 000 U/ml时,能够显著改变CD4+CD25+Treg细胞的低增殖能力,而且IL-2与IL-4的共同作用改变更明显,其中高浓度的IL-2能够阻断CD4+CD25+Treg细胞对CD4+CD25-T细胞的抑制功能。结论慢性丙型肝炎患者外周血中CD4+CD25+Treg细胞的抑制活性增强,高浓度IL-2可阻断CD4+CD25+Treg细胞的抑制效应。Objective To study the role of CD4^＋CD25^＋ T cells in CD4^＋ T cell responses from persistent hepatitis C virus infected patients and the effect of exogenous IL-2 on inhibitory capacity of CD4^＋CD25^＋ T cells. Methods CD4^＋CD25^＋ T cells were isolated from 18 chronic hepatitis C patients and 15 healthy donors by immunomagentic beads. To assess the regulatory properties of CD4^＋CD25^＋ T cells, CD4^＋CD25^＋ T cells were cocultured with CD4^＋CD25^- T cells from patients or controls, then IL-2 and IL-4 were added to cultures. Results Chronic hepatitis C patients exhibited an increased inhibitory capacity （P = 0.034） of CD4^＋CD25^＋ T cells, CD4^＋CD25^＋ T cells dramatically suppress the proliferation of CD4^＋ T cells; IL-2 enhanced the proliferation of CD4^＋CD25^＋ T cells only at high concentration （2000 U/ml） and IL-4 had a similar effect. When two cytokines were mixed, they had strong synergistic effects. High concen- trations of IL-2 could break CD4^＋CD25^＋ T cells anergy as well as overcoming its regulatory activity. Conclusions Persistent hepatitis C virus infected patients show an increased immunosuppressive capacity of CD4^＋CD25^＋ Treg cells in the peripheral blood and IL-2 could break its regulatory activity.

关 键 词：丙型肝炎病毒 CD4+CD25+TREG细胞 IL-2 

分 类 号：R512.6[医药卫生—内科学]